SPARC is a major secretory gene expressed and involved in the development of proliferative diabetic retinopathy.
SPARC is a major secretory gene expressed and involved in the development of proliferative diabetic retinopathy.
复制标题
DOI：
10.5551/jat.e711
复制
发表时间：
2009-04
影响因子：
4.4
通讯作者：
Kazuhisa Watanabe;F. Okamoto;Tomotaka Yokoo;K. Iida;Hiroaki Suzuki;H. Shimano;T. Oshika;N. Yamada;H. Toyoshima
中科院分区：
文献类型：
--
作者：
Kazuhisa Watanabe;F. Okamoto;Tomotaka Yokoo;K. Iida;Hiroaki Suzuki;H. Shimano;T. Oshika;N. Yamada;H. Toyoshima
目的新生血管是增殖性糖尿病视网膜病变（proliferative diabetic retinopathy，PDR）的重要病理过程，包括多种生长因子在内的多种分泌蛋白参与了这一过程。我们寻找从PDR患者的眼睛获得的手术标本中表达的分泌蛋白。方法我们开发了寡帽信号序列陷阱（SST）策略，使我们能够从最小的起始材料筛选分泌或膜蛋白。使用这种方法，我们能够筛选从PDR患者的眼睛获得的手术标本构建的cDNA文库。结果所克隆的cDNA大多数编码富含半胱氨酸的酸性分泌蛋白，表明半胱氨酸在PDR中高表达。对来自不同患者的玻璃体液的分析表明，PDR患者中β蛋白的浓度增加。此外，视网膜下注射重组腺病毒可诱导大鼠眼内PDR样改变。结论：我们的研究结果强烈地表明，糖尿病视网膜病变（DR）的发展中所涉及的。
AIM Neovascularization is an important event in proliferative diabetic retinopathy (PDR), where various secretory proteins including multiple growth factors are considered to be involved in this process. We searched for secretory proteins expressed in a surgical specimen obtained from the eyes of patients with PDR. METHODS We developed the oligo-cap signal sequence trap (SST) strategy which enables us to screen for secretory or membrane proteins from a minimal starting material. Using this method, we were able to screen a cDNA library constructed from a surgical specimen obtained from the eyes of the patients with PDR. RESULTS Majority of the cloned cDNAs turned out to encode secreted protein acidic and rich in cystein (SPARC), strongly suggesting that SPARC is highly expressed in PDR. Analysis of vitreous fluid from various patients has shown that the concentration of SPARC protein is increased in patients with PDR. Furthermore, subretinal injection of recombinant SPARC adenovirus induced PDR-like changes in the rat eye. CONCLUSIONS Our results strongly suggested that SPARC is involved in the development of diabetic retinopathy (DR).