Phylogenetic analysis of Plectosphaerella species based on multi-locus DNA sequences and description of P. sinensis sp nov.
Phylogenetic analysis of Plectosphaerella species based on multi-locus DNA sequences and description of P. sinensis sp nov.
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DOI：
10.1007/s11557-017-1319-8
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发表时间：
2017-08-01
影响因子：
2.4
通讯作者：
Niu, Yong-Chun
中科院分区：
文献类型：
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作者：
Su, Lei;Deng, Hui;Niu, Yong-Chun
Plectosphaerella中的物种是众所周知的几种植物的病原体，引起果实、根和颈腐烂和塌陷。通过对我国葫芦科植物内生真菌的调查，分离到77株属于Plectosphaerella属的内生真菌。为了对分离菌株进行鉴定，我们收集了除口虾蛄外的所有目前公认的Plectosphaerella种的模式菌株或参考菌株，并进行了系统发育分析。28 S rDNA部分序列的系统发育分析表明，丛毛壳菌属的所有种均属于丛毛壳科的一个分支。基于ITS、CaM、EF1、TUB和形态学特征的多位点系统发育分析表明，所有种均得到了较好的分离。从中国北方的南瓜、西瓜和梅洛茎中分离到3株内生真菌，命名为中国南瓜内生真菌（P.sinensis）。该新种与其他Plectosphaerella种的形态不同之处在于不规则的厚垣孢子，以及瓶梗和分生孢子的尺寸。从几种瓜类植物中分离到的其他内生菌株被鉴定为黄瓜疫霉。
Species in Plectosphaerella are well known as pathogens of several plant species causing fruit, root and collar rot and collapse. In an investigation of endophytic fungi associated with cucurbit plants in China, we isolated 77 strains belonging to the genus Plectosphaerella. To identify the isolated strains, we collected the type or reference strains of all currently accepted species in Plectosphaerella except P. oratosquillae and conducted a phylogenetic analysis. Phylogenetic analysis of the partial 28S rDNA sequences showed that all species in Plectosphaerella were located in one clade of Plectosphaerellaceae. Based on multi-locus phylogenetic analysis of the ITS, CaM, EF1, TUB and morphological characteristics, all species in Plectosphaerella were well separated. Three endophytic strains from stems of Cucurbita moschata, Citrullus lanatus and Cucumis melo from North China were assigned to a new species described as P. sinensis in this paper. The new species differs morphologically from other Plectosphaerella species by irregular chlamydospores, and the dimensions of phialides and conidia. The other endophytic strains from several cucurbit plants were identified as P. cucumerina.