Hydrogen production by immobilized R. faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria E. harbinense B49
Hydrogen production by immobilized R. faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria E. harbinense B49
复制标题
利用乙醇发酵细菌哈尔滨大肠杆菌 B49 的可溶性代谢物固定化粪肠杆菌 RLD-53 产氢
DOI:
10.1016/j.biortech.2008.12.020
复制
发表时间:
2009-05-01
影响因子:
11.4
通讯作者:
Xie, Guo-Jun
中科院分区:
文献类型:
--
作者:
Liu, Bing-Feng;Ren, Nan-Qi;Xie, Guo-Jun
In order to increase the hydrogen yield from glucose, hydrogen production by immobilized Rhodopseudomonas faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria Ethanoligenens harbinense B49 was investigated. The soluble metabolites from dark-fermentation mainly were ethanol and acetate, which could be further utilized for photo-hydrogen production. Hydrogen production by B49 was noticeably affected by the glucose and phosphate buffer concentration. The maximum hydrogen yield (1.83 mol H-2/mol glucose) was obtained at 9 g/l glucose. In addition, we found that the ratio of acetate/ethanol (A/E) increased with increasing phosphate buffer concentration, which is favorable to further photo-hydrogen production. The total hydrogen yield during dark- and photo-fermentation reached its Maximum Value (6.32 mol H-2/mol glucose) using 9 g/l glucose, 30 mmol/l phosphate buffers and immobilized R. faecalis RLD-53. Results demonstrated that the combination of dark- and photo-fermentation was an effective and efficient process to improve hydrogen yield from a single substrate. (C) 2008 Elsevier Ltd. All rights reserved.