Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties
Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties
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DOI：
10.1016/j.foodres.2017.09.073
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发表时间：
2017-12-01
影响因子：
8.1
通讯作者：
Jose Galotto, Maria
中科院分区：
文献类型：
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作者：
Lopez de Dicastillo, Carol;Bustos, Fernanda;Jose Galotto, Maria
研究了毛果乌尔塔(Ugni molinae Turcz.)一直由智利本土文化拥有。本研究对不同提取物的酚类含量、抗氧化性、抗菌活性和抗酪氨酸酶活性进行了研究，以探讨其在食品、医药和化妆品方面的应用前景。在水、乙醇和50%乙醇的条件下提取穆尔塔果实和叶。通过Flin Ciocalteu法测定提取物中酚类物质的含量，并用ORAC、FRAP、DPPH和TEAC四种不同的抗氧化剂体系测定其抗氧化能力，以阐明其主要的抗氧化机理。通过高效液相色谱法对芦丁、异槲皮苷和水合物中的一些黄酮类成分进行了鉴定和定量。用最小抑菌浓度(MIC)和最小杀菌浓度(MBC)测定了它们对大肠杆菌和单核细胞增多性李斯特菌的抑菌活性，并用流式细胞术证实了它们对单核细胞增多性李斯特菌的抑制作用。水提物中多酚类化合物的含量最高(28+/-1 mg没食子酸/g干果，128+/-6 mg/g干叶)。生物学特性的值也有相同的趋势：水乙醇提取物显示出最强的活性。树叶表现出比穆尔塔水果更高的抗氧化、抗微生物和抗酪氨酸酶特性。根据ORAC、FRAP、TEAC和DPPH计算的最高抗氧化活性，Murta果实样品分别为80+/-8 mg Trolox/g、70+/-2 mg Trolox/g、87+/-8 mg Trolox/g和110+/-12 mg Trolox/g；Murta叶分别为280+/-10 mg Trolox/g、192+/-4 mg Trolox/g、286+/-13 mg Trolox/g和361+/-13 mg Trolox/g。这些活性得到了高效液相分析的证实，表明所分析的化合物在树叶水醇萃取物中含量最高。在抑菌作用方面，水提物的活性最高，对单核细胞增多性李斯特氏菌的MIC值最低(0.07 mg/mL)。该提取物还具有最高的抗酪氨酸酶活性(叶和果实的CE50值分别为1.6+/-0.3(g/L)和8.9+/-1.2(g/L))。
The knowledge of the biological properties of fruits and leaves of murta (Ugni molinae Turcz.) has been owned by native Chilean culture. The present study investigated the phenolic content, the antioxidant, antimicrobial and anti-tyrosinase activities of different murta fruit and leaves extracts to approach their uses on future food, pharmaceutical and cosmetic applications. Extractions of murta fruit and leaves were carried out under water, ethanol and ethanol 50%. Phenolic content of these extracts was measured through Folin Ciocalteu test and the antioxidant power by four different antioxidant systems (ORAC, FRAP, DPPH and TEAC assays) owing to elucidate the main mechanism of antioxidant. Some flavonoids, such as rutin, isoquercitrin and quercitrin hydrate were identified and quantified through HPLC analysis. Antimicrobial activity was determined measuring minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) values against Escherichia coil and Listeria monocytogenes, and the effect of these extracts on L. monocytogenes was confirmed by flow cytometry.Highest contents of polyphenol compounds were obtained in hydroalcoholic extracts (28 +/- 1 mg gallic acid/g dry fruit, and 128 +/- 6 mg gallic acid/g dry leaves). The same trend was found for the values of biological properties: hydroalcoholic extracts showed the strongest activities. Leaves presented higher antioxidant, antimicrobial and anti-tyrosinase properties than murta fruit. Highest antioxidant activity values according to ORAC, FRAP, TEAC and DPPH were 80 +/- 8 mg Trolox/g, 70 +/- 2 mg Trolox/g, 87 +/- 8 mg Trolox/g and 110 +/- 12 mg Trolox/g, respectively, for murta fruit samples, and 280 +/- 10 mg Trolox/g, 192 +/- 4 mg Trolox/g, 286 +/- 13 mg Trolox/g and 361 +/- 13 mg Trolox/g, respectively, for murta leaves. These activities were confirmed by HPLC analysis that revealed highest presence of analyzed compounds on leaves hydroalcoholic extract. Regarding to antimicrobial analysis, hydroalcoholic leaves extract presented the highest activity presenting the lowest MIC value for L. monocytogenes (0.07 mg/mL). This extract also performed the highest anti-tyrosinase activity (CE50 values of 1.6 +/- 0.3 (g/L) and 8.9 +/- 1.2 (g/L) for leaves and fruit, respectively).