Antioxidant activities of water-soluble polysaccharide extracted from mung bean (Vigna radiata L.) hull with ultrasonic assisted treatment
Antioxidant activities of water-soluble polysaccharide extracted from mung bean (Vigna radiata L.) hull with ultrasonic assisted treatment
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超声辅助处理绿豆壳水溶性多糖的抗氧化活性
DOI：
10.1016/j.carbpol.2010.02.011
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发表时间：
2010-06-11
影响因子：
11.2
通讯作者：
Li, Xiaofeng
中科院分区：
文献类型：
--
作者：
Lai, Furao;Wen, Qibiao;Li, Xiaofeng
研究了绿豆（Vigna radiata L）壳中水溶性多糖（MSP）的超声波辅助提取及其抗氧化活性。脱蛋白后，MSP通过DEAE-纤维素阴离子交换柱和Sephadex G-100凝胶渗透柱进行分离纯化。通过 FT-IR、GC 和凝胶渗透色谱 (GPC) 对两种酸性多糖组分 MP1 和 MP2 进行了表征。结果表明，MP1和MP2为酸性杂多糖，糖醛酸含量分别为9.9%和36.4%。 MP1的主要成分是甘露糖，MP2的主要成分是鼠李糖和半乳糖。 MP1和MP2的分子量分别为83 kDa和45 kDa。虽然MP2表现出较高的羟自由基清除活性，但MP1较MP2表现出较高的还原力、更强的超氧自由基和DPPH自由基清除能力以及对1,2,3-苯三醇自氧化的抑制作用。因此，MP1 应该作为一种潜在的抗氧化剂进行探索。 (C) 2010 Elsevier Ltd. 保留所有权利。
Ultrasonic assisted extraction and antioxidant activity of water-soluble polysaccharide (MSP) from mung bean (Vigna radiata L) hull were investigated. After deproteinization, MSP was isolated and purified by a DEAE-cellulose anion-exchange column and a Sephadex G-100 gel-permeation column. Two acid polysaccharide fractions, MP1 and MP2, were characterized by FT-IR, GC, and gel-permeation chromatography (GPC). Results indicated that MP1 and MP2 were acid heteropolysaccharide with 9.9% and 36.4% of uronic acid contents respectively. The main composition of MP1 was mannose, whereas MP2 consisted of rhamnose and galactose. The molecule weights of MP1 and MP2 were 83 kDa and 45 kDa, respectively. Although, MP2 showed higher hydroxyl radical-scavenging activity, MP1 exhibited higher reducing power and stronger scavenging capacity for superoxide radical and DPPH radicals as well as higher inhibition on self-oxidation of 1,2,3-phentriol than that of MP2. Thus, MP1 should be explored as a potential antioxidant. (C) 2010 Elsevier Ltd. All rights reserved.