Activity, abundance and structure of ammonia-oxidizing microorganisms in plateau soils
Activity, abundance and structure of ammonia-oxidizing microorganisms in plateau soils
复制标题
高原土壤氨氧化微生物的活性、丰度和结构
DOI：
10.1016/j.resmic.2015.07.012
复制
发表时间：
2015-10-01
影响因子：
2.6
通讯作者：
Liu, Yong
中科院分区：
文献类型：
--
作者：
Dai, Yu;Wu, Zhen;Liu, Yong
氨氧化古菌（AOA）和细菌（AOB）都可以参与土壤生态系统中氨向亚硝酸盐的生物转化。然而，高原土壤中AOA和AOB的分布及影响因素尚不清楚。本研究分别采用潜在硝化率（PNR）、定量PCR测定和克隆文库分析评估了云南高原不同土壤中氨氧化剂的活性、丰度和结构。高原土壤中 AOA 和 AOB 群落存在较大差异。 PNR 与 AOB 丰度呈显着正相关。两者都是通过有机碳与氮的比率（C/N）和总磷（TP）来确定的。 AOB在氨氧化中可以发挥更重要的作用。 AOB 群落多样性可能受土壤总氮 (TN) 和总有机碳 (TOC) 的影响，并且通常高于 AOA 群落多样性。此外，亚硝化球菌和亚硝化螺菌类生物分别是高原土壤中的主要 AOA 和 AOB。 AOA 群落结构可能由 TP 和 C/N 决定，而 AOB 群落结构由 pH 值决定。 (c) 2015 年巴斯德研究所。由 Elsevier Masson SAS 出版。版权所有。
Both ammonia-oxidizing archaea (AOA) and bacteria (AOB) can be involved in biotransformation of ammonia to nitrite in soil ecosystems. However, the distribution of AOA and AOB in plateau soils and influential factors remain largely unclear. In the present study, the activity, abundance and structure of ammonia oxidizers in different soils on the Yunnan Plateau were assessed using potential nitrification rates (PNRs), quantitative PCR assay and clone library analysis, respectively. Wide variation was found in both AOA and AOB communities in plateau soils. PNRs showed a significant positive correlation with AOB abundance. Both were determined by the ratio of organic carbon to nitrogen (C/N) and total phosphorous (TP). AOB could play a more important role in ammonia oxidation. AOB community diversity was likely affected by soil total nitrogen (TN) and total organic carbon (TOC) and was usually higher than AOA community diversity. Moreover, Nitrososphaera- and Nitrosospira-like organisms, respectively, were the dominant AOA and AOB in plateau soils. AOA community structure was likely shaped by TP and C/N, while AOB community structure was determined by pH. (c) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.