Establishment of the mesodermal cell line QCE-6. A model system for cardiac cell differentiation.
Establishment of the mesodermal cell line QCE-6. A model system for cardiac cell differentiation.
复制标题
中胚层细胞系QCE-6的建立。
DOI：
10.1161/01.res.78.2.205
复制
发表时间：
1996
影响因子：
20.1
通讯作者：
Bader,DM
中科院分区：
文献类型：
--
作者：
Eisenberg,CA;Bader,DM
QCE-6细胞系来源于日本鹌鹑心前中胚层。如前所述，这些细胞能够分化为具有心肌或心内膜内皮细胞特性的两种不同的心脏细胞类型。本文详细描述了该细胞系的起源，并进一步描述了这些细胞的未处理和诱导心肌和内皮表型。QCE-6细胞表现出具有心脏前中胚层特征的上皮形态和蛋白表达模式。视黄酸、碱性成纤维细胞生长因子（bFGF）、转化生长因子(TGF)-β2和TGF-β3诱导这些细胞分化并产生上皮细胞和间充质细胞的混合培养。上皮细胞在细胞质中以点状模式表达肌球蛋白、聚乳酸蛋白和心肌肌钙蛋白I。当TGF-β1、血小板衍生生长因子(PDGF)-BB和胰岛素样生长因子（IGF） II与视黄酸、bFGF、TGF-β2和TGF-β3联合加入时，这些肌合成蛋白以肌原纤维前模式组织。此外，这些处理诱导Na+，K+- atp酶的表达。当QCE-6细胞在I型胶原上培养时，视黄酸、bFGF、TGF-β2、TGF-β3促进间充质细胞侵入凝胶。这些间充质细胞QH1和JB3阳性，这两种细胞都是早期心源性中胚层内心内膜细胞的标记物。在QCE-6细胞培养中加入PDGF-BB和IGF II将抑制视黄酸、bFGF、TGF-β2和TGF-β3诱导间质形态和QH1和JB3表达的能力。总之，这些结果表明，心脏细胞分化过程受多种信号调节，早期心源性中胚层含有一种双电位干细胞，可以产生心肌和心内膜谱系。更重要的是，由于QCE-6细胞是早期心源性细胞的代表，该细胞系为研究心脏细胞分化提供了独特的模型系统。
The QCE-6 cell line was derived from precardiac mesoderm of the Japanese quail. As previously reported, these cells are able to differentiate into two distinct cardiac cell types with myocardial or endocardial endothelial cell properties. This present communication describes in detail the derivation of this cell line and further characterizes the nontreated and induced myocardial and endothelial phenotypes of these cells. The QCE-6 cells exhibit an epithelial morphology, as well as the pattern of protein expression, that is characteristic of precardiac mesoderm. Treatment with retinoic acid, basic fibroblast growth factor (bFGF), transforming growth factor (TGF)-β2, and TGF-β3 induces these cells to differentiate and produce mixed cultures of epithelial and mesenchymal cells. The epithelial cells express myosin, desmin, and cardiac troponin I in a punctate pattern throughout the cytoplasm. These sarcomeric proteins become organized in a premyofibrillar pattern when TGF-β1, platelet-derived growth factor (PDGF)-BB, and insulin-like growth factor (IGF) II are added in combination along with retinoic acid, bFGF, TGF-β2, and TGF-β3. Also, these treatments induce Na+,K+-ATPase expression. When the QCE-6 cells are cultured on collagen type I, the mesenchymal cells that are promoted by retinoic acid, bFGF, TGF-β2, and TGF-β3 will invade the gel. These mesenchymal cells are positive for QH1 and JB3, which are both markers for presumptive endocardial cells within the early cardiogenic mesoderm. The addition of both PDGF-BB and IGF II to QCE-6 cell cultures will inhibit the ability of retinoic acid, bFGF, TGF-β2, and TGF-β3 to induce both the mesenchymal morphology and QH1 and JB3 expression. Collectively, these results suggest that the process of cardiac cell differentiation is regulated by multiple signals and that early cardiogenic mesoderm contains a bipotential stem cell that can give rise to both the myocardial and endocardial lineages. More important, since the QCE-6 cells are representative of early cardiogenic cells, this cell line offers a unique model system to study cardiac cell differentiation.