Structural Dynamics Measurements of the Biological Molecule Using Pump-Probe Technique
Structural Dynamics Measurements of the Biological Molecule Using Pump-Probe Technique
复制标题
使用泵探针技术测量生物分子的结构动力学
DOI：
10.5940/jcrsj.56.253
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
N. Shibayama
中科院分区：
文献类型：
--
作者：
A. Tomita;S. Nozawa;S. Adachi;Tokushi Sato;N. Shibayama
for the of the dynamics动态.本文介绍了利用同步辐射源和脉冲激光系统产生的单束团X射线进行亚纳秒级时间分辨的泵浦探测方法。二聚血红蛋白的时间分辨溶液揭示了两个亚基和三个中间状态的螺旋运动。在蛋白质动力学的其他测量方法中，泵浦-探测方法更是一种强有力的技术。
for the of the dynamics. we introduce the pump-probe method using combined single bunch X-ray from synchrotron source and pulsed laser system in sub nano-second time resolution. Time-resolved solution of dimeric hemoglobin revealed a spiral motion of two subunits and three intermediate states. The pump-probe method is even powerful technique in the other methods of measurement for the protein dynamics.