Odorant receptor (OR) gene choice is biased and non-clonal in two olfactory placode cell lines, and OR RNA is nuclear prior to differentiation of these lines.
Odorant receptor (OR) gene choice is biased and non-clonal in two olfactory placode cell lines, and OR RNA is nuclear prior to differentiation of these lines.
复制标题
在两个嗅基板细胞系中，气味受体 (OR) 基因的选择是有偏向的且非克隆的，并且 OR RNA 在这些细胞系分化之前是核的。
DOI：
10.1111/j.1471-4159.2008.05780.x
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发表时间：
2009
影响因子：
4.7
通讯作者：
Lane,RobertP
中科院分区：
文献类型：
--
作者：
Pathak,Nidhi;Johnson,Paul;Getman,Michael;Lane,RobertP
我们研究了两个克隆小鼠嗅觉基板（OP）细胞系作为研究内源性气味受体（OR）调控的模型系统。这两种细胞系都可以分化为双极神经元，其转录谱与成熟的感觉神经元一致。我们发现单细胞在体内表现出与感觉神经元一样的单基因OR表达。单基因OR表达在未分化的细胞中建立，并在分化过程中持续存在，但OR基因的选择并不是两种细胞系的克隆特性。有趣的是，在两种细胞系的分化过程中，OR RNA主要从核转移到细胞质。最后，我们的数据表明，在细胞群体中，OR基因和OR簇的有限子集被过度代表，这表明，要么是OP创始细胞中预先存在的内在偏见，要么是由培养条件引起的外在影响。
We have investigated two clonal mouse olfactory placode (OP) cell lines as a model system for studying endogenous odorant receptor (OR) regulation. Both lines can be differentiated into bipolar neurons with transcriptional profiles consistent with mature sensory neurons. We show that single cells exhibit monogenic OR expression like sensory neuronsin vivo. Monogenic OR expression is established in undifferentiated cells and persists through differentiation, but OR gene choice is not a clonal property of either cell line. Interestingly, OR RNA shifts from predominantly nuclear to cytoplasma during differentiation of both cell lines. Finally, our data indicate that a restricted subset of OR genes and OR clusters are over‐represented in cell populations, suggesting either a pre‐existing intrinsic bias in OP founder cells or extrinsic influences arising from culture conditions.