Phylogenetic relationships of Pythium species based on ITS and 5.8S sequences of the ribosomal DNA
Phylogenetic relationships of Pythium species based on ITS and 5.8S sequences of the ribosomal DNA
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DOI：
10.1007/bf02463876
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发表时间：
1999-08-15
期刊：
影响因子：
1.4
通讯作者：
Hyakumachi, Mitsuro
中科院分区：
文献类型：
--
作者：
Matsumoto, Chieko;Kageyama, Koji;Hyakumachi, Mitsuro
解析了腐霉属 30 个物种和两个类群的 ITS 区域序列。在系统发育树中，该物种通常分为两个簇，此处称为 F 组和 S 组。两组物种的孢子囊形态一致，F 组为丝状/分叶状，S 组为球形。 F组内的遗传分化低于S组内的遗传分化。其他形态特征，例如卵子结构和性本质似乎与这些树的分组无关。比对分析揭示了所有物种的共同序列以及每个 F 或 S 组特有的排列。结果发现，ITS 区域是设计用于鉴定和检测腐霉属物种的物种特异性引物的良好目标。在基于 5.8S rDNA 序列的树中，卵菌与其他真菌关系较远，但与 Chromista 中的藻类分离。
The sequences of ITS regions in 30 species and two groups of the genus Pythium were resolved. In the phylogenetic trees, the species were generally divided into two clusters, referred to here as the F and S groups. The species in the two groups correspond in terms of their sporangial morphology, with the F group being filamentous/lobulate and the S group being spherical. Genetic divergence within the F group was lower than that within the S group. Other morphological characteristics such as oogonial structure and sexual nature appeared to be unrelated to the groupings in these trees. An alignment analysis revealed common sequences to all the species and arrangements specific to each F or S group. It was found that the ITS region was a good target in designing species-specific primers for the identification and detection of Pythium species. In the tree based on 5.8S rDNA sequences, oomycetes are distantly related to other fungi but separated from algae in Chromista.