An ultraviolet fluorescence-based method for identifying and distinguishing protein crystals
An ultraviolet fluorescence-based method for identifying and distinguishing protein crystals
复制标题
DOI：
10.1107/s0907444904026538
复制
发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
González, C
中科院分区：
文献类型：
--
作者：
Judge, RA;Swift, K;González, C
本征紫外荧光作为一种快速、无损伤的蛋白质晶体鉴定方法已得到广泛的研究。一个落射荧光显微镜，它提供了从上面的样品，和一个直通的几何形状，它提供了从上面的激发和从下面的样品的荧光视图的荧光的激发和查看，与蛋白质和非蛋白质晶体样品进行了测试。在这两个系统中，观察到蛋白质晶体发出明亮的荧光，提供了对背景溶液荧光的高对比度，从而使蛋白质晶体被识别和区分从非蛋白质晶体。
Intrinsic ultraviolet fluorescence has been investigated as a rapid non-invasive method for identifying and distinguishing protein crystals. An epi-fluorescence microscope, which provides for excitation and viewing of fluorescence from above the sample, and a straight-through geometry, which provides excitation from above and views fluorescence from underneath the sample, were tested with protein and nonprotein crystal samples. In both systems the protein crystals were observed to fluoresce brightly, providing a high contrast against background solution fluorescence, thus enabling protein crystals to be identified and distinguished from nonprotein crystals.