Combination of multiple mRNA markers (PTTG1, survivin, UbcH10 and TK1) in the diagnosis of Taiwanese patients with breast cancer by membrane array
Combination of multiple mRNA markers (PTTG1, survivin, UbcH10 and TK1) in the diagnosis of Taiwanese patients with breast cancer by membrane array
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DOI：
10.1159/000098557
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发表时间：
2006-01-01
期刊：
影响因子：
3.5
通讯作者：
Lin, Shiu-Ru
中科院分区：
文献类型：
--
作者：
Chen, Chung-Chi;Chang, Tsai-Wang;Lin, Shiu-Ru
目的：早期发现是有效降低乳腺癌发病率和死亡率的先决条件。本研究旨在采用高通量膜阵列来检测女性乳腺癌患者外周血中循环肿瘤细胞（CTC）表达的一组 mRNA 标记物。方法：采集92例乳腺癌患者和100例正常人的外周血。使用膜阵列技术检测CTC。使用的标记物包括垂体瘤转化基因1、生存素、UbcH10和胸苷激酶1。 结果：结果显示，在乳腺癌细胞稀释实验中，膜阵列每1ml外周血能够阳性检测到5个癌细胞。对于 4 种 mRNA 标记物组，敏感性和特异性分别提高至 86% 和 88%。此外，发现患者的临床病理特征肿瘤大小（p = 0.006）、组织学分级（p = 0.012）、淋巴结转移（p = 0.001）和TNM分期（p = 0.006）与多标志物组的阳性检出率显着相关。结论：这些结果表明，我们的多标记膜阵列方法可以以相当高的灵敏度和特异性检测乳腺癌患者循环中的 CTC。版权所有 (c) 2006 S. Karger AG，巴塞尔
Objective: Early detection is a prerequisite to the effective reduction of morbidity and mortality from breast cancer. The present study intended to employ a high-throughput membrane array to detect a panel of mRNA markers expressed by circulating tumor cells (CTCs) in the peripheral blood of female patients with breast cancer. Methods: Peripheral blood was sampled from 92 breast cancer patients and 100 normal persons. CTCs were detected by using a membrane array technique. The markers used included the pituitary tumor transforming gene 1, survivin, UbcH10 and thymidine kinase 1. Results: The results showed that the membrane array could positively detect 5 cancer cells per 1 ml of peripheral blood in breast cancer cell dilution experiments. For the panel of 4 mRNA markers, sensitivity and specificity were elevated up to 86 and 88%, respectively. Furthermore, it was found that the patients' clinicopathological characteristics tumor size (p = 0.006), histologic grade (p = 0.012), lymph node metastasis (p = 0.001) and TNM stage (p = 0.006) significantly correlated with the positive detection rate of the multimarker panel. Conclusions: These findings demonstrated that our multimarker membrane array method could detect CTCs in the circulation of breast cancer patients with considerably high sensitivity and specificity. Copyright (c) 2006 S. Karger AG, Basel