TISSUE-LEVELS OF BONE RESORPTIVE CYTOKINES IN PERIODONTAL-DISEASE
TISSUE-LEVELS OF BONE RESORPTIVE CYTOKINES IN PERIODONTAL-DISEASE
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DOI：
10.1902/jop.1991.62.8.504
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发表时间：
1991-08-01
影响因子：
4.3
通讯作者：
SOCRANSKY, SS
中科院分区：
文献类型：
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作者：
STASHENKO, P;JANDINSKI, JJ;SOCRANSKY, SS
在牙周病部位的组织中评估3种骨吸收细胞因子，白细胞介素1-α（IL-1-α）、IL-1-β和肿瘤坏死因子-α（TNF-α）的水平。如通过组织提取物的ELISA所确定的，在所有患病部位中检测到IL-1-β和TNF-α，而IL-1-α存在于8/22个部位中。IL-1-β的浓度最高（平均值+/- SEM：11，695 +/-2，888 pg/ml; 672 pM），其次是TNF-α（434 +/- 135 pg/ml; 26 pM）和IL-1-α（342 +/- 160 pg/ml; 20 pM）。所有3种介质的水平在临床健康组织中均显著较低。IL-1-β和TNF-α水平之间存在高度显著的相关性（r（s）= 0.61，P < 0.001），提示这两种介质的协调表达。还通过冷冻组织切片上的间接免疫荧光测定含有每种介质的细胞的数量。与组织提取物的结果一致，含IL-1-β的细胞的数量比含TNF-α的细胞高约5倍，比含IL-1-α的细胞高40倍。与以前的研究结果一起，这些结果表明IL-1-β可能是牙周病发病机制中的重要介质。
THE LEVELS OF 3 BONE RESORPTIVE CYTOKINES, interleukin 1-alpha (IL-1-alpha), IL-1-beta, and tumor necrosis factor-alpha (TNF-alpha), were assessed in tissues from sites of periodontal disease. As determined by ELISA of tissue extracts, IL-1-beta and TNF-alpha were detected in all diseased sites, whereas IL-1-alpha was present in 8/22 sites. IL-1-beta was present in highest concentration (mean +/- SEM: 11,695 +/- 2,888 pg/ml; 672 pM), followed by TNF-alpha (434 +/- 135 pg/ml; 26 pM), and IL-1-alpha (342 +/- 160 pg/ml; 20 pM). The levels of all 3 mediators were significantly lower in clinically healthy tissues. There was a highly significant correlation between levels of IL-1-beta and TNF-alpha (r(s) = 0.61, P < 0.001), suggesting coordinated expression of these 2 mediators. The numbers of cells containing each mediator was also determined by indirect immunofluorescence on frozen tissue sections. Consistent with findings from tissue extracts, IL-1-beta-containing cells were present in approximately 5-fold higher numbers than TNF-alpha-containing cells, and 40-fold higher numbers than IL-1-alpha-containing cells. Taken together with previous findings, these results indicate that IL-1-beta is likely to be an important mediator in the pathogenesis of periodontal disease.