The Arabidopsis WAVY GROWTH 2 protein modulates root bending in response to environmental stimuli
The Arabidopsis WAVY GROWTH 2 protein modulates root bending in response to environmental stimuli
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DOI：
10.1105/tpc.104.028530
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发表时间：
2005-02-01
期刊：
影响因子：
11.6
通讯作者：
Sakai, T
中科院分区：
文献类型：
--
作者：
Mochizuki, S;Harada, A;Sakai, T
为了了解根的生长方向如何随着障碍物、光照和重力的变化而变化，我们对拟南芥突变体Wavy Growth 2(WAVE 2)进行了表征，该突变体的根在倾斜的琼脂介质上显示出短节距的波状生长模式。在波状生长、向重力和向光性反应中，波2突变体的根弯曲程度比野生型幼苗大。Wave2-1根表皮波状生长模式的细胞文件旋转在右手旋转和左手旋转中都有所增加。WAV2编码一个属于芽涌现46家族的蛋白，其N端有一个跨膜区，C端有一个α/β水解酶结构域。表达分析表明，WAV2在成年植株的根和幼苗中表达较强，尤其是在根尖、细胞延伸区和中柱中表达较强。我们的结果表明，WAV2不参与感知环境刺激，但它通过抑制根尖旋转来负向调节刺激诱导的根弯曲。
To understand how the direction of root growth changes in response to obstacles, light, and gravity, we characterized an Arabidopsis thaliana mutant, wavy growth 2 (wav2), whose roots show a short-pitch pattern of wavy growth on inclined agar medium. The roots of the wav2 mutant bent with larger curvature than those of the wild-type seedlings in wavy growth and in gravitropic and phototropic responses. The cell file rotations of the root epidermis of wav2-1 in the wavy growth pattern were enhanced in both right-handed and left-handed rotations. WAV2 encodes a protein belonging to the BUD EMERGENCE 46 family with a transmembrane domain at the N terminus and an alpha/beta-hydrolase domain at the C terminus. Expression analyses showed that mRNA of WAV2 was expressed strongly in adult plant roots and seedlings, especially in the root tip, the cell elongation zone, and the stele. Our results suggest that WAV2 is not involved in sensing environmental stimuli but that it negatively regulates stimulus-induced root bending through inhibition of root tip rotation.