Transcriptome and phenotypic responses of Vibrio cholerae to increased cyclic di-GMP level
Transcriptome and phenotypic responses of Vibrio cholerae to increased cyclic di-GMP level
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DOI：
10.1128/jb.188.10.3600-3613.2006
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发表时间：
2006-05-01
影响因子：
3.2
通讯作者：
Yildiz, Fitnat H.
中科院分区：
文献类型：
--
作者：
Beyhan, Sinem;Tischler, Anna D.;Yildiz, Fitnat H.
霍乱弧菌是霍乱的病原体，是一种兼性的人类病原体，具有肠道和水生生命周期。霍乱弧菌识别和响应环境中波动参数的能力对其生存至关重要。在许多微生物中，第二信使3‘，5’-环二鸟氨酸(c-di-GMP)被认为是整合影响细胞生理的环境刺激的重要因素。对霍乱弧菌基因组的序列分析表明，编码具有GGDEF结构域和/或EAL结构域的蛋白的基因非常丰富，预计这些基因可以调节细胞内c-di-GMP的浓度。为了阐明c-di-GMP控制的细胞过程，测定了El Tor霍乱弧菌和经典霍乱弧菌生物型对c-di-GMP浓度升高的全基因组转录组反应。结果表明，霍乱弧菌对c-di-GMP水平升高的反应是通过增加vps、eps和msh基因的转录，而降低鞭毛基因的转录。霍乱弧菌中其他c-di-GMP调控基因的功能尚不清楚。
Vibrio cholerae, the causative agent of cholera, is a facultative human pathogen with intestinal and aquatic life cycles. The capacity of V. cholerae to recognize and respond to fluctuating parameters in its environment is critical to its survival. In many microorganisms, the second messenger, 3',5'-cyclic diguanylic acid (c-di-GMP), is believed to be important for integrating environmental stimuli that affect cell physiology. Sequence analysis of the V. cholerae genome has revealed an abundance of genes encoding proteins with either GGDEF domains, EAL domains, or both, which are predicted to modulate cellular c-di-GMP concentrations. To elucidate the cellular processes controlled by c-di-GMP, whole-genome transcriptome responses of the El Tor and classical V. cholerae biotypes to increased c-di-GMP concentrations were determined. The results suggest that V. cholerae responds to an elevated level of c-di-GMP by increasing the transcription of the vps, eps, and msh genes and decreasing that of flagellar genes. The functions of other c-di-GMP-regulated genes in V. cholerae are yet to be identified.