A novel vehicle-like drug delivery 3D printing scaffold and its applications for a rat femoral bone repairing in vitro and in vivo
A novel vehicle-like drug delivery 3D printing scaffold and its applications for a rat femoral bone repairing in vitro and in vivo
复制标题
一种新型类载体药物输送3D打印支架及其在体外和体内大鼠股骨修复中的应用
DOI：
10.7150/ijbs.37552
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发表时间：
2020-01-01
影响因子：
9.2
通讯作者：
Zeng, Xiangqiong
中科院分区：
文献类型：
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作者：
Wang, Hui;Deng, Zhengwei;Zeng, Xiangqiong
介孔生物活性玻璃（MBG）的高比表面积和特殊结构赋予了其对各种药物优良的物理吸附性，同时又不破坏其化学活性。硅酸盐1393生物活性玻璃（1393）以其优异的生物降解性和成骨性而闻名。在此，我们通过在1393（1393@MBG）表面涂覆MBG，构建了具有多重药物递送能力的新型载体状药物递送3D打印支架。此外，我们在1393@MBG支架上应用了DEX和BMP-2，以赋予其抗菌和成骨特性。结果表明，1393@MBG支架能有效地负载和控制释放BMP-2、DNA和DEX，可用于骨科治疗。体外抗菌实验表明，载药1393@MBG对金黄色葡萄球菌（Staphylococcus aureus，S. BMP-2负载1393@MBG可提高人骨髓间充质干细胞（hBMSCs）碱性磷酸酶（ALP）活性，上调成骨相关基因（OCN和RUNX 2）的表达。此外，体内研究进一步证实，在大鼠股骨缺损中，与1393组相比，负载BMP-2的1393@MBG组显示出更好的成骨能力。总之，这些结果表明，载体样药物递送3D打印支架1393@MBG可能是骨修复和相关骨疾病治疗的有希望的候选者。
The high surface area ratio and special structure of mesoporous bioactive glass (MBG) endow it with excellent physical adsorption of various drugs without destroying the chemical activity. Silicate 1393 bioactive glass (1393) is famous for its fantastic biodegradability and osteogenesis. Herein, we have built a novel vehicle-like drug delivery 3D printing scaffold with multiplexed drug delivery capacity by coating MBG on the surface of 1393 (1393@MBG). Furthermore, we have applied DEX and BMP-2 on the 1393@MBG scaffold to endow it with antibacterial and osteogenic properties. Results indicated that this 1393@MBG scaffold could effectively load and controlled release BMP-2, DNA and DEX, which can be applied for orthopedic treatment. The in vitro study showed that the DEX loaded 1393@MBG exhibited excellent antibacterial ability, which was evaluated by Staphylococcus aureus (S. aureus), and the BMP-2 loaded 1393@MBG can improve the alkaline phosphatase (ALP) activity and upregulate the expression of osteogenesis-related genes (OCN and RUNX2) of human bone mesenchymal stem cells (hBMSCs). Moreover, the in vivo study further confirmed that the BMP-2 loaded 1393@MBG group showed better osteogenic capacity as compared to that of the 1393 group in a rat femoral defect. Together, these results suggested that the vehicle-like drug delivery 3D printing scaffold 1393@MBG could be a promising candidate for bone repair and relative bone disease treatment.