A NOVEL HOMEOBOX NKCH4 GENE FROM THE DROSOPHILA 93E REGION
A NOVEL HOMEOBOX NKCH4 GENE FROM THE DROSOPHILA 93E REGION
复制标题
DOI：
10.1016/0378-1119(93)90715-f
复制
发表时间：
1993-05-30
期刊：
影响因子：
3.5
通讯作者：
BELLARD, M
中科院分区：
文献类型：
--
作者：
JAGLA, K;GEORGEL, P;BELLARD, M
我们克隆了一个果蝇同源异型盒基因，该基因定位于第三条染色体右臂上的93 D9-E2带，位于NK同源异型盒基因簇的近端区域。与NK-1和其他9个已知的同源异型盒基因一样，nkch 4（NK簇同源异型盒4）在Glu 44和Val 45的同源异型域密码子之间含有内含子。nkch 4同源结构域序列与人类HOX 11（tcl 3）T细胞癌基因的同源性最高（57%），但与所有其他同源框基因的同源结构域的第三螺旋中的几个保守残基不同，已知对DNA识别很重要。低水平的nkch 4转录本检测到胚胎发生的后期阶段，以及在三龄幼虫和蛹。在晚期胚胎中，nkch 4在发育中的CNS中表达。
We have cloned a Drosophilia melanogaster homeobox gene that maps to bands 93D9-E2 on the right arm of the third chromosome, in the proximal region of the NK-homeobox gene cluster. Like NK-1 and nine other known homeobox genes, nkch4 (NK-cluster homeobox 4) contains an intron between the homeodomain codons for Glu44 and Val45. The nkch4 homeodomain sequence is most related to that of the human HOX11 (tcl3) T-cell oncogene (57% homology), but differs from all other homeobox genes at several conserved residues in the third helix of the homeodomain, known to be important for DNA recognition. Low levels of nkch4 transcripts were detected during late stages of embryogenesis as well as in third instar larvae and pupae. In late embryos nkch4 is expressed in the developing CNS.