The effect of extracellular pH changes on intracellular pH and nitric oxide concentration in endothelial and smooth muscle cells from rat aorta.
The effect of extracellular pH changes on intracellular pH and nitric oxide concentration in endothelial and smooth muscle cells from rat aorta.
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DOI：
10.1371/journal.pone.0062887
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Celotto AC
中科院分区：
文献类型：
--
作者：
Capellini VK;Restini CB;Bendhack LM;Evora PR;Celotto AC
世纪以来，人们都知道pH值的变化可以改变血管张力。然而，关于pH变化对血管反应的影响尚无共识。在这项研究中，我们研究了细胞外pH值（pHo）的变化对细胞内pH值（pHi）和细胞内一氧化氮浓度（[NO]i）在新鲜分离的内皮细胞和横截面从大鼠主动脉的影响。使用HCl将pH从7.4降低至7.0和从7.4降低至6.5;使用NaOH将pH从7.4增加至8.0和从7.4增加至8.5。荧光染料5-（和-6）-羧基SNARF-1、乙酰氧基甲酯乙酸酯（SNARF-1）和二氨基荧光素-FM二乙酸酯（DAF-FM DA）分别用于测定pHi和[NO]i。通过流式细胞术在新鲜分离的内皮细胞中测量荧光强度，并通过共聚焦显微镜在新鲜获得的主动脉横截面中测量荧光强度。在pHo 8.5时，内皮和血管平滑肌pHi增加。细胞外酸化并没有改变内皮细胞的pHi，但平滑肌的pHi降低在pHo 7.0。在pHo 8.5和pHo 6.5时，内皮细胞[NO]i增加。细胞外碱化和酸化均增加血管平滑肌[NO]i。并不是所有的pH值的变化都导致pHi的变化，但酸碱平衡在两个方向上的破坏诱导内皮细胞和/或血管平滑肌中的NO合成。
It has been known for more than a century that pH changes can alter vascular tone. However, there is no consensus about the effects of pH changes on vascular response. In this study, we investigated the effects of extracellular pH (pHo) changes on intracellular pH (pHi) and intracellular nitric oxide concentration ([NO]i) in freshly isolated endothelial cells and cross sections from rat aorta. The HCl was used to reduce the pHo from 7.4 to 7.0 and from 7.4 to 6.5; the NaOH was used to increase the pHo from 7.4 to 8.0 and from 7.4 to 8.5. The fluorescent dyes 5-(and-6)-carboxy SNARF-1, acetoxymethyl ester, acetate (SNARF-1) and diaminofluorescein-FM diacetate (DAF-FM DA) were employed to measure the pHi and [NO]i, respectively. The fluorescence intensity was measured in freshly isolated endothelial cells by flow cytometry and in freshly obtained aorta cross sections by confocal microscopy. The endothelial and vascular smooth muscle pHi was increased at pHo 8.5. The extracellular acidification did not change the endothelial pHi, but the smooth muscle pHi was reduced at pHo 7.0. At pHo 8.5 and pHo 6.5, the endothelial [NO]i was increased. Both extracellular alkalinization and acidification increased the vascular smooth muscle [NO]i. Not all changes in pHo did result in pHi changes, but disruption of acid-base balance in both directions induced NO synthesis in the endothelium and/or vascular smooth muscle.
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影响因子：
6.1
作者：
Hattori, K;Tsuchida, S;Muramatsu, I
通讯作者：
Muramatsu, I
影响因子：
20.1
作者：
Niwa, K;Haensel, C;Iadecola, C
通讯作者：
Iadecola, C
影响因子：
5
作者：
Celotto, Andrea C.;Restini, Carolina B. A.;Evora, Paulo R. B.
通讯作者：
Evora, Paulo R. B.
影响因子：
3.9
作者：
Celotto, A. C.;Capellini, V. K.;Evora, P. R. B.
通讯作者：
Evora, P. R. B.
影响因子：
7.3
作者：
MITCHELL, JA;DENUCCI, G;VANE, JR
通讯作者：
VANE, JR