Synthesis of quantum dot encoded multicolour nanobeads for the ultrasensitive and multiplex immunochromatographic detection of neonicotinoid insecticides
Synthesis of quantum dot encoded multicolour nanobeads for the ultrasensitive and multiplex immunochromatographic detection of neonicotinoid insecticides
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DOI：
10.1016/j.snb.2022.131671
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发表时间：
2022-03
期刊：
影响因子：
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通讯作者：
H. Cao;Shujie Cao;Y. Han;Weiyi Zhang;Ziqi Wei;Tai Ye;Min Yuan;Jin-song Yu;Xiuxiu Wu;
中科院分区：
文献类型：
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作者：
H. Cao;Shujie Cao;Y. Han;Weiyi Zhang;Ziqi Wei;Tai Ye;Min Yuan;Jin-song Yu;Xiuxiu Wu;
在本研究中，开发了一种基于量子点编码多色纳米珠（QB）的新型荧光免疫色谱分析（ICA）试纸条，用于同时检测蔬菜样品中的三种新烟碱类杀虫剂。结果表明，通过将不同比例的两种疏水性 CdSe/ZnS 量子点（λem= 565 nm 和 λem= 620 nm）嵌入聚合物基体中，成功合成了具有红光、黄光和绿光发射的三种 QB。获得的三个 QB 进一步用于标记吡虫啉 (IMI)、噻虫胺 (CLT) 和噻虫嗪 (TMX) 特异性的单克隆抗体 (mAb)。所得的 QB@mAbs 纳米探针，包括 QBred@IMI mAbs、QByellow@CLT mAbs 和 QBgreen@TMX mAbs，呈现出优异的单分散性和可区分的发射颜色。此外，基于 QB@mAbs 纳米探针制作的 QB-ICA 试纸条实现了对三种新烟碱类杀虫剂的更灵敏的同时检测，IMI 的检测限为 14.58 ng/mL，CLT 的检测限为 0.27 ng/mL，TMX 的检测限为 0.36 ng/mL。这比大多数传统的酶联免疫吸附测定 (ELISA) 更好。此外，基于 QB 的 ICA 试纸条对于 IMI、CLT 和 TMX 的多重检测也表现出良好的稳定性和特异性。加标大白菜样品中，IMI、CLT、TMX 的回收率为 72.08%～98.70%，RSD 值为 3.60%～13.09%。简而言之，所开发的 QB-ICA 方法的出色性能证明了其在农产品中同时定量新烟碱类杀虫剂检测的潜力。
In this study, a novel fluorescent immunochromatographic assay (ICA) strip based on quantum dot encoded multicolour nanobead (QB) was developed to detect three neonicotinoid insecticides in the vegetable samples simultaneously. The results showed that three QBs with red-, yellow- and green-emitting were successfully synthesized by embedding different ratios of two kinds of hydrophobic CdSe/ZnS QDs (λem= 565 nm and λem= 620 nm) into the polymer matrix. The obtained three QBs were further used to label the monoclonal antibodies (mAbs) specific to imidacloprid (IMI), clothianidin (CLT) and thiamethoxam (TMX). The resultant QB@mAbs nanoprobes, including QBred@IMI mAbs, QByellow@CLT mAbs and QBgreen@TMX mAbs, presented excellent monodispersity with distinguishable emission colours. Furthermore, the fabricated QB-ICA strip based on QB@mAbs nanoprobes achieved a more sensitive simultaneous detection for three neonicotinoids with the detection limits of 14.58 ng/mL for IMI, 0.27 ng/mL for CLT, and 0.36 ng/mL for TMX, respectively. This was better than most of the conventional based enzyme-linked immunosorbent assay (ELISA). Moreover, the QB-based ICA strips also exhibited good stability and specificity for the multiplex detection of IMI, CLT, and TMX. In the spiked Chinese cabbage samples, the recovery of IMI, CLT, and TMX ranged from 72.08% to 98.70%, with RSD values ranging from 3.60% to 13.09%. In brief, the great performance of the developed QB-ICA approach demonstrates its potential for simultaneous and quantitative neonicotinoids detection in agricultural products.