Any Way You Slice It-A Comparison of Confocal Microscopy Techniques.
Any Way You Slice It-A Comparison of Confocal Microscopy Techniques.
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DOI：
10.7171/jbt.15-2602-003
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发表时间：
2015-07-01
期刊：
影响因子：
--
通讯作者：
Brown, Claire M
中科院分区：
文献类型：
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作者：
Jonkman, James;Brown, Claire M
共聚焦荧光显微镜已经成为生命科学研究人员的一种流行工具，主要是因为它能够去除图像焦平面外的模糊。已经开发了几种不同类型的共焦显微镜，每种都有优缺点。本文将介绍栅格共聚焦、经典共聚焦激光扫描显微镜(CLSM)、共振扫描激光扫描显微镜和旋转圆盘共聚焦显微镜。将介绍每种显微镜技术的工作方式，该技术适合的最佳应用，该技术的局限性，以及每种技术的新发展。能够接触到一系列不同的共焦显微镜(例如，通过本地核心设施)的研究人员应该会发现，这篇论文有助于为特定的成像应用选择最佳的共焦技术。其他有资金购买仪器的人应该会发现，这篇文章有助于决定哪种技术最适合他们的研究领域。
The confocal fluorescence microscope has become a popular tool for life sciences researchers, primarily because of its ability to remove blur from outside of the focal plane of the image. Several different kinds of confocal microscopes have been developed, each with advantages and disadvantages. This article will cover the grid confocal, classic confocal laser-scanning microscope (CLSM), the resonant scanning-CLSM, and the spinning-disk confocal microscope. The way each microscope technique works, the best applications the technique is suited for, the limitations of the technique, and new developments for each technology will be presented. Researchers who have access to a range of different confocal microscopes (e.g., through a local core facility) should find this paper helpful for choosing the best confocal technology for specific imaging applications. Others with funding to purchase an instrument should find the article helpful in deciding which technology is ideal for their area of research.