Expression profiles of active genes in human and mouse livers
Expression profiles of active genes in human and mouse livers
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DOI：
10.1016/0378-1119(96)00512-4
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发表时间：
1996-09-26
期刊：
影响因子：
3.5
通讯作者：
Matsubara, K
中科院分区：
文献类型：
--
作者：
Kawamoto, S;Matsumoto, Y;Matsubara, K
通过使用忠实代表 mRNA 群体组成的 3' 定向 cDNA 文库收集序列，获得了人类肝脏中活性基因的表达谱。结果显示约的相对活性。 600 个基因维持肝细胞并维持其肝脏特异性表型。最活跃的基因组是那些用于产生血浆蛋白的基因，其次是用于合成脂蛋白、蛋白酶抑制剂、凝血因子和补体的基因。从人类肝脏（包括成人和胎儿的肝脏）中独立获得的四个表达谱保持了这种基因活性的平衡。将表达谱扩展到成年小鼠的肝脏，用作肝细胞分子病因学和检查药物作用的模型。人类和小鼠肝脏之间细微的生物学差异反映在活性基因的整体表达谱上，特别是在血浆蛋白、脂蛋白和补体的合成方面。这种使用表达谱的比较分析应该在比较生物学中得到广泛的应用。
An expression profile of active genes in the human liver was obtained by collecting sequences with a 3'-directed cDNA library that faithfully represents composition of the mRNA population. The results show the relative activity of ca. 600 genes in maintaining the hepatocytes and sustaining their liver-specific phenotypes. The most active group of genes are those for the production of plasma proteins, followed by the genes for the synthesis of lipoproteins, protease inhibitors, coagulation factors, and complements. This balance of gene activity was maintained for four independently obtained expression profiles from human livers, including those of adult and fetus. The expression profiling was extended to the liver of adult mouse, used as a model for the molecular etiology of hepatocytes and for examining the effects of drugs. Subtle biological differences between the human and mouse livers are reflected in the global expression profiles of active genes, especially with regard to the synthesis of plasma proteins, lipoproteins and complements. This comparative analysis using expression profiling should find a wide application in comparative biology.