Hypersensitive to red and blue 1, a ZZ-type zinc finger protein, regulates phytochrome B-Mediated red and cryptochrome-mediated blue light responses
Hypersensitive to red and blue 1, a ZZ-type zinc finger protein, regulates phytochrome B-Mediated red and cryptochrome-mediated blue light responses
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DOI：
10.1105/tpc.104.029165
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发表时间：
2005-03-01
期刊：
影响因子：
11.6
通讯作者：
Ni, M
中科院分区：
文献类型：
--
作者：
Kang, XJ;Chong, J;Ni, M
调控光致光发育的植物光感受器包括吸收红光/远红光的光敏色素和吸收蓝光/ uv -a光的隐色素。我们进行了遗传筛选，以确定拟南芥光感受器下游的其他成分。我们在红蓝光下发现了一个短的下胚轴突变体，对红蓝1 （hrb1）敏感。HRB1突变还增强了日末远红光响应，抑制了叶片扩张和叶柄伸长，减弱了CAB3和CHS的表达。双突变体分析表明，在红光下，phyB对hrb I具有上位性，cry1 cry2对hrb 1具有蓝光下的上位性，对下胚轴生长和光调控基因的表达均有响应。HRB1定位于细胞核，属于干旱诱导19 （Di19）蛋白家族。HRB1和所有其他家族成员都含有一个zz型锌指结构域，在其他生物体中，该结构域与肌营养不良蛋白和钙调蛋白之间以及转录衔接子和激活子之间的蛋白相互作用有关。HRB1活性也是红光和蓝光诱导光敏色素相互作用因子4 （PIF4）表达所必需的。Pif4对红光和蓝光都表现出与HRBL非常相似的超敏反应，并且在控制光调节基因表达反应方面对HRBL具有上位性。因此，HRB1和PIF4共同调控红光和蓝光响应的作用可能代表了红光信号和蓝光信号的交叉点。
Plant photoreceptors that regulate photornorphogenic development include red/far-red-light-absorbing phytochromes and blue/UV-A-light-absorbing cryptochromes. We have undertaken a genetic screen to identify additional components downstream of the photoreceptors in Arabidopsis thaliana. We identified a short hypocotyl mutant under red and blue light, hypersensitive to red and blue 1 (hrb1). Mutation in HRB1 also enhances the end-of-day far-red light response, inhibits leaf expansion and petiole elongation, and attenuates the expression of CAB3 and CHS. Double mutant analysis indicates that phyB is epistatic to hrb I under red light, and cry1 cry2 is epistatic to hrb 1 under blue light for both hypocotyl growth and light-regulated gene expression responses. HRB1 localizes to the nucleus and belongs to a protein family of Drought induced 19 (Di19). HRB1 and all other family members contain a ZZ-type zinc finger domain, which in other organisms is implicated in protein-protein interactions between dystrophin and calmodulin and between transcriptional adaptors and activators. HRB1 activity is also required for red and blue light-induced expression of PHYTOCHROME INTERACTING FACTOR 4 (PIF4). pif4 shows a very similar hypersensitive response as hrbl to both red light and blue light and is epistatic to hrbl in control of light-regulated gene expression responses. Thus, the roles of HRB1 and PIF4 together in regulating both red and blue light responses may represent points where red light signaling and blue light signaling intersect.