Maternal nutrition alters the expression of insulin-like growth factors in fetal sheep liver and skeletal muscle
Maternal nutrition alters the expression of insulin-like growth factors in fetal sheep liver and skeletal muscle
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DOI：
10.1677/joe.0.1670429
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发表时间：
2000-12-01
影响因子：
4
通讯作者：
Symonds, ME
中科院分区：
文献类型：
--
作者：
Brameld, JM;Mostyn, A;Symonds, ME
我们研究了妊娠第28天和第80天之间的母亲饮食限制，然后重新喂养至妊娠140天（期限为147天）的绵羊中的良好喂养的母羊的摄入量，对胰岛素样生长因子（IGF）-I，IGF-II和生长激素受体（GHR）在胎儿肝脏和骨骼肌中的mRNA表达的影响。在妊娠第28天和第80天之间，单胎母羊消耗3.2-3.8 MJ/天的代谢能（ME）（即营养限制-约为ME需求的60%，考虑到母羊维持和孕体生长的需求）或8.7-9.9 MJ/天（即喂养良好-约为ME需求的150%）。然后，所有欧盟的良好喂养（150%的ME需求），直到妊娠第140天，消耗8-10.9兆焦耳/天。在妊娠第80天和第140天，从每组中取样5只母羊，并采集胎仔组织。在采样日期或骨骼肌时，组间胎仔体重或肝脏重量存在Ilo差异（四头肌）重量：在140天。IGF-I mRNA丰度较低的肝脏营养限制的胎儿在妊娠80天（营养限制2.35;喂养良好的3.70任意单位），但高于妊娠第140天喂养良好的胎儿，60天后重新喂养（限制/重新喂养4.27;营养良好的2.83; S.E.D. 0.98任意单位，对于饮食X年龄相互作用，P=0.061）。IGF-II mRNA丰度在营养限制胎儿的肝脏中始终较高（80天：营养限制7.78;良好喂养5.91; 140天：限制/重新喂养7.23;良好喂养6.01; S.E.D. 1.09任意单位，对于饮食，P=0.061）。营养限制对肝脏GHR mRNA丰度没有影响，但与连续良好喂养的胎儿相比，先前营养限制的胎儿重新喂养增加了GHR mRNA（80天：营养限制70.6;良好喂养75.1; 140天：限制/重新喂养115.7;良好喂养89.4，S.E.D.）。10.13任意单位，对于饮食x年龄相互作用，P = 0.047）。胎儿骨骼肌IGF-I mRNA丰度不受母体营养的影响，随孕龄增加而降低（P
We investigated the influence of maternal dietary restriction between days 28 and 80 of gestation followed by re-feeding to the intake of well-fed ewes up to 140 days of gestation (term is 147 days) in sheep, on expression of mRNA for insulin-like growth factor (IGF)-I, IGF-II and growth hormone receptor (GHR) in fetal liver and skeletal muscle. Singleton bearing ewes either consumed 3.2-3.8 MJ/day of metabolisable energy (ME) (i.e. nutrient restricted - approximately 60% of ME requirements, taking into account requirements for both ewe maintenance and growth of the conceptus) or 8.7-9.9 MJ/day (i.e. well fed - approximately 150% of ME requirements) between days 28 and 80 of gestation. All eu es were then well fed (150% of ME requirements) up to day 140 of gestation and consumed 8-10.9 MJ/day. At days 80 and 140 of gestation, five ewes were sampled from each group and fetal tissues taken. There was Ilo difference in fetal body weight or liver weights between groups at either sampling date, or skeletal muscle (quadriceps) weight: at 140 days.IGF-I mRNA abundance was lower in livers of nutrient-restricted fetuses at day 80 of gestation (nutrient restricted 2.35; well fed 3.70 arbitrary units), but was higher than well-fed fetuses at day 140 of gestation, after 60 days of re-feeding (restricted/re-fed 4.27; well fed 2.83; S.E.D. 0.98 arbitrary units, P=0.061 for diet x age interaction). IGF-II mRNA abundance was consistently higher in livers of nutrient-restricted fetuses (80 clays: nutrient restricted 7.78; well fed 5.91; 140 days: restricted/re-fed 7.23; well fed 6.01; S.E.D. 1.09 arbitrary units, P=0.061 for diet). Nutrient restriction had no effect on hepatic GHR mRNA abundance, hut re-feeding of previously nutrient-restricted fetuses increased GHR mRNA compared with continuously well-fed fetuses (80 days: nutrient restricted 70.6; well fed 75.1; 140 days: restricted/re-fed 115.7; well fed 89.4, S.E.D. 10.13 arbitrary units, P = 0.047 for diet x age interaction). In fetal skeletal muscle, IGF-I mRNA abundance was not influenced by maternal nutrition and decreased with gestation age (P