Colonisation and infection of the paranasal sinuses in cystic fibrosis patients is accompanied by a reduced PMN response
Colonisation and infection of the paranasal sinuses in cystic fibrosis patients is accompanied by a reduced PMN response
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DOI：
10.1016/j.jcf.2012.04.011
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发表时间：
2012-12-01
影响因子：
5.2
通讯作者：
von Buchwald, Christian
中科院分区：
文献类型：
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作者：
Johansen, Helle Krogh;Aanaes, Kasper;von Buchwald, Christian
背景：我们研究了鼻窦是否可能是铜绿假单胞菌肺部感染的病灶。方法：对78例CF患者行鼻内窥镜手术，用PFGE进行细菌基因分型。取自鼻窦和肺部的材料进行革兰氏染色以检测生物被膜。结果：从鼻窦和肺部同时培养铜绿假单胞菌时，40例患者中有38例(95%)基因完全一致。在鼻窦中，铜绿假单胞菌形成生物膜，细胞炎症程度最低，可能是因为局部分泌性IgA的产生明显高于免疫球蛋白(p
Background: We studied whether the sinuses might be foci for Pseudomonas aeruginosa lung infection.Methods: Endoscopic Sinus Surgery was performed in 78 CF patients; PFGE was used for bacterial genotyping. Material from sinuses and lungs were Gram-stained to detect biofilms. Immunoglobulins were measured in serum and saliva.Results: When P. aeruginosa was cultured simultaneously from die sinuses and the lungs they were genetically identical in 38 of the 40 patients (95%). In the sinuses, P. aeruginosa formed biofilms with minimal cellular inflammation, probably because of a significantly higher local production of secretory IgA compared with IgG (p