Structure of oligosaccharide side chains of an intestinal immune system modulating arabinogalactan isolated from rhizomes of Atractylodes lancea DC
Structure of oligosaccharide side chains of an intestinal immune system modulating arabinogalactan isolated from rhizomes of Atractylodes lancea DC
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DOI：
10.1016/j.carres.2004.01.003
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发表时间：
2004-03-15
影响因子：
3.1
通讯作者：
Yamada, H
中科院分区：
文献类型：
--
作者：
Taguchi, I;Kiyohara, H;Yamada, H
已发现在兰芝根茎中存在调节肠道免疫系统的阿拉伯-3，6-半乳聚糖（ALR-5IIa-1-1）。[Planta Medica 1998，64，714-719; Carbohydr. Polyms 2001，46，147-156]，然而，来自落叶松属和金合欢属的其它阿拉伯-3，6-半乳聚糖未能表达调节活性。用内切-β-D-（1-> 6）-半乳聚糖酶降解Araf侧链修剪的ALR-5IIa-1-1（AF-ALR-5IIa-1-1）中的半乳糖基侧链显著降低AF-ALR-5IIa-1-1的活性。结构分析表明，ALR-5IIa-1-1中主要的内切-β-D-（1-→ 6）-半乳聚糖酶稳定侧链由d.p.1 -8的β-D-（1-→ 6）-吡喃半乳糖基寡糖组成。由于AF-ALR-5IIa-1-1中β-D-（1 - 3）-半乳聚糖主链的降解也显著降低了其活性，因此认为这些连接到β-D-（1 - 3）半乳聚糖主链上的半乳糖基侧链中的一些负责ALR-5IIa-1-1活性的表达。(C)2004爱思唯尔有限公司保留所有权利。
An intestinal immune system modulating arabino-3,6-galactan (ALR-5IIa-1-1) has been found in rhizomes of Atractylodes lancea DC. [Planta Medica 1998, 64, 714-719; Carbohydr. Polyms. 2001, 46, 147-156], however other arabino-3,6-galactans from Larix and Acacia failed to express the modulating activity. Degradation of the galactosyl side chains in Araf-side chain-trimmed ALR-5IIa-1-1 (AF-ALR-5IIa-1-1) with an endo-beta-D-(l --> 6)-galactanase remarkably decreased the activity of AF-ALR5IIa-1-1. Structural analysis indicated that the major endo-beta-D-(l --> 6)-galactanase-digestable side chains in ALR-5IIa-1-1 are composed Of beta-D-(l --> 6)-galactopyranosyl oligosaccharides having d.p. 1-8. Because degradation of the beta-D-(l --> 3)-galactan backbone in AF-ALR-5IIa-1-1 also significantly reduced its activity, some of these galactosyl side chains attached to beta-D-(I --> 3)galactan backbone are suggested to be responsible for expression of the activity of ALR-5IIa-1-1. (C) 2004 Elsevier Ltd. All rights reserved.