Bone Marrow Mesenchymal Stem Cells Increase Survival after Ionizing Irradiation Combined with Wound Trauma: Characterization and Therapy.
Bone Marrow Mesenchymal Stem Cells Increase Survival after Ionizing Irradiation Combined with Wound Trauma: Characterization and Therapy.
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DOI：
10.4172/2157-7013.1000190
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Gorbunov, Nikolai V
中科院分区：
文献类型：
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作者：
Kiang, Juliann G;Gorbunov, Nikolai V
本研究的目的是探讨间充质干细胞（MSC）治疗是否可以提高放射复合损伤后的生存率。从B6D2F1/J雌性小鼠股骨中分离骨髓间充质干细胞（BMSCs），并在低氧条件（5% O2、10% CO2、85% N2）下扩增培养30天。由于 60Co-γ-光子照射（9.25 和 9.75 Gy，0.4 Gy/min，双侧）联合损伤后 24 小时，将 BMSC 输注至小鼠，然后进行皮肤损伤 (CI)。在观察期间监测耗水量、体重、伤口愈合和存活率。在 30 天的观察期内，接受 CI 治疗的小鼠经历了戏剧性的濒临死亡。因此，CI (9.25 Gy)-动物组的特点是死亡率为40%，而CI (9.75 Gy)-动物组的死亡率为100%。 CI 引起的疾病伴随着体重减轻、饮水量增加和伤口愈合延迟。受伤后第 30 天，存活的 CI 小鼠中仍然存在骨髓细胞缺失。用 BMSC 治疗 CI (9.25 Gy) 动物组可使 30 天存活率提高 30%，减轻体重，加速伤口愈合速度，并改善骨髓细胞耗竭。我们的新结果首次表明 BMSC 疗法可有效维持 CI 后动物的生存。
The aim of this study was to investigate whether treatment with mesenchymal stem cells (MSCs) could improve survival after radiation combined injury. Bone marrow MSCs (BMSCs) were isolated from femurs of B6D2F1/J female mice and were expanded and cultivated in hypoxic conditions (5% O2, 10% CO2, 85% N2) over 30 days. BMSCs were transfused to mice 24 hr after combined injury due to 60Co-gamma-photon irradiation (9.25 and 9.75 Gy, 0.4 Gy/min, bilateral) followed by skin wounding (CI). Water consumption, body weight, wound healing, and survival tallies were monitored during observation period. Mice subjected to CI experienced a dramatic moribundity over a 30-day observation period. Thus, CI (9.25 Gy)-animal group was characterized by 40% mortality rate while CI (9.75 Gy)-animal group had 100% mortality rate. CI-induced sickness was accompanied by body weight loss, increased water intake, and delayed wound healing. At the 30th day post-injury, bone marrow cell depletion still remained in surviving CI mice. Treatment of CI (9.25 Gy)-animal group with BMSCs led to an increase in 30-day survival rate by 30%, attenuated body weight loss, accelerated wound healing rate, and ameliorated bone-marrow cell depletion. Our novel results are the first to suggest that BMSC therapy is efficacious to sustain animal survival after CI.