Induction of Th17 cells by segmented filamentous bacteria in the murine intestine.
Induction of Th17 cells by segmented filamentous bacteria in the murine intestine.
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DOI：
10.1016/j.jim.2015.03.020
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发表时间：
2015-06
影响因子：
2.2
通讯作者：
Ivanov II
中科院分区：
文献类型：
--
作者：
Farkas AM;Panea C;Goto Y;Nakato G;Galan-Diez M;Narushima S;Honda K;Ivanov II
分节丝状细菌（SFB）是革兰氏阳性、厌氧、孢子形成的共生菌，存在于许多动物的肠道中。四十多年前，SFB被描述为通过诱导IgA和Th17细胞调节宿主免疫系统的独特能力，最近引起了人们的兴趣。在这里，我们描述了一系列检测和量化肠粘膜中SFB和SFB粘附以及固有层中SFB特异性CD4 T细胞的方法。此外，我们还描述了从SFB单相关的无菌小鼠粪便中纯化SFB的方法。利用这些方法，我们研究了SFB定植和Th17细胞诱导的动力学。我们还表明，SFB在肠粘膜上的定植不均匀，并且SFB粘附主要发生在回肠末端，并与SFB特异性Th17细胞的增加相关。
Segmented filamentous bacteria (SFB) are gram positive, anaerobic, spore-forming commensals that reside in the gut of many animal species. Described more than forty years ago, SFB have recently gained interest due to their unique ability to modulate the host immune system through induction of IgA and Th17 cells. Here, we describe a collection of methods to detect and quantify SFB and SFB adhesion in intestinal mucosa, as well as SFB-specific CD4 T cells in the lamina propria. In addition, we describe methods for purification of SFB from fecal material of SFB-monoassociated gnotobiotic mice. Using these methods we examine the kinetics of SFB colonization and Th17 cell induction. We also show that SFB colonize unevenly the intestinal mucosa and that SFB adherence occurs predominantly in the terminal ileum and correlates with an increased representation of SFB-specific Th17 cells.