Intelligent self-control of carbon metabolic flux in SecY-engineered Escherichia coli for xylitol biosynthesis from xylose-glucose mixtures
Intelligent self-control of carbon metabolic flux in SecY-engineered Escherichia coli for xylitol biosynthesis from xylose-glucose mixtures
复制标题
SecY工程大肠杆菌中碳代谢通量的智能自控用于从木糖-葡萄糖混合物生物合成木糖醇
DOI：
10.1002/bit.28002
复制
发表时间：
--
影响因子：
3.8
通讯作者：
Li Deng
中科院分区：
文献类型：
--
作者：
Qiang Guo;Irfan Ullah;Ling‐Jie Zheng;Xin‐Quan Gao;Chen‐Yang Liu;Hui‐Dong Zheng;Li‐Hai Fan;Li Deng
木糖醇是一种有益的糖替代品，已广泛用于食品，制药和化学工业。通过代谢工程细胞工厂共发酵木糖和葡萄糖是木糖化学氢化用于木糖醇商业生产的有前景的替代方案。在此，我们在产木糖醇的大肠杆菌JM 109（DE 3）中工程改造了SecY蛋白转运通道（SecY [ΔP]）的突变体，作为木糖摄取的通道。发现SecY（ΔP）通道可以快速转运木糖而不受XylB催化的木糖醇磷酸合成的干扰，这对于天然XylFGH和XylE转运蛋白是不可能的。更重要的是，在SecY（ΔP）通道和碳代谢阻遏（CCR）的共同作用下，木糖向磷酸戊糖（PP）途径和木糖醇合成途径的流动在E.大肠杆菌可以响应于葡萄糖而自动控制，从而确保突变细胞能够以高木糖醇产量充分利用糖。你.本研究开发的colicell工厂已被证明适用于广泛的木糖-葡萄糖混合物，这有利于简化混合糖发酵过程，以高效经济地生产木糖醇。
Xylitol is a salutary sugar substitute that has been widely used in the food, pharmaceutical, and chemical industries. Co‐fermentation of xylose and glucose by metabolically engineered cell factories is a promising alternative to chemical hydrogenation of xylose for commercial production of xylitol. Here, we engineered a mutant of SecY protein‐translocation channel (SecY [ΔP]) in xylitol‐producingEscherichia coliJM109 (DE3) as a passageway for xylose uptake. It was found that SecY (ΔP) channel could rapidly transport xylose without being interfered by XylB‐catalyzed synthesis of xylitol‐phosphate, which is impossible for native XylFGH and XylE transporters. More importantly, with the coaction of SecY (ΔP) channel and carbon catabolite repression (CCR), the flux of xylose to the pentose phosphate (PP) pathway and the xylitol synthesis pathway inE. colicould be automatically controlled in response to glucose, thereby ensuring that the mutant cells were able to fully utilize sugars with high xylitol yields. TheE. colicell factory developed in this study has been proven to be applicable to a broad range of xylose‐glucose mixtures, which is conducive to simplifying the mixed‐sugar fermentation process for efficient and economical production of xylitol.