In-vitro flower formation in leaf explants of tomato: effect of NaCl
In-vitro flower formation in leaf explants of tomato: effect of NaCl
复制标题
番茄叶外植体的体外成花：氯化钠的影响
作者：
Kebin Liu;Shu
以普通番茄（Lycopersicon esculentum Mill.）和ofL.茴芹属在含有不同浓度NaCl的Murashige-Skoog培养基上培养。11个基因型的培养物在含0.5%NaCl的培养基上均能形成花芽。花的形成发生直接分化的叶培养或由再生芽的顶端从营养状态到花芽的过渡。在不含NaCl或含1.0%NaCl以上的培养基上均无花形成。不同基因型番茄叶片离体成花能力存在较大差异。外植体形成花的基因型均为有限生长型。
Leaf explants of 24 cultivars and 2 F1 hybrids of the common tomato (Lycopersicon esculentum Mill.) and ofL. pimpinellifoliumBrezh. were cultured on Murashige-Skoog medium containing different concentrations of NaCl. The cultures of 11 genotypes formed flower buds when cultured on medium containing 0.5% NaCl. Flower formation occurred either by direct differentiation from the leaf cultures or by transition of the apices of regenerated shoots from the vegetative state to floral buds. No flower formation occurred on medium without NaCl or media with 1.0% NaCl or more. There existed great differences in the capacity of in-vitro flower formation in the tomato leaf explants among the genotypes tested. The genotypes whose explants did form flowers were all of determinate growth habit.