CALCIUM AND STIMULUS-SECRETION COUPLING IN MAST-CELL - STIMULANT AND INHIBITORY EFFECTS OF CALCIUM-RICH MEDIA ON EXOCYTOSIS
CALCIUM AND STIMULUS-SECRETION COUPLING IN MAST-CELL - STIMULANT AND INHIBITORY EFFECTS OF CALCIUM-RICH MEDIA ON EXOCYTOSIS
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DOI：
10.1113/jphysiol.1977.sp011976
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发表时间：
1977-01-01
影响因子：
5.5
通讯作者：
KAGAYAMA, M
中科院分区：
文献类型：
--
作者：
DOUGLAS, WW;KAGAYAMA, M
分离的大鼠腹腔肥大细胞在无钙培养基中孵育2小时，有或没有EDTA，并通过相差显微镜观察，成为起泡的外观时，随后暴露于培养基中富含钙（16-110 mM）。EM显示反应是由常规肥大细胞促分泌素如抗原（在致敏细胞中）和48/80引起的复合胞吐。对钙的反应被抑制撤回葡萄糖和添加二硝基苯酚，因此是能量依赖性的。同样高浓度的镁对钙剥夺细胞没有这种刺激作用，而过量的钙只在钙剥夺后才刺激。Ca剥夺可增加肥大细胞质膜的渗透性，从而当随后以高浓度引入时允许一些Ca渗透并激活胞吐作用。这一结果可能进一步支持Ca在刺激-分泌偶联中的介导作用。检测到高浓度Ca的两种抑制作用：抑制细胞内胞吐凹中颗粒的迁移或排出;以及降低对48/80 [4-甲氧基-N-甲基-苯乙胺和甲醛的缩合产物]的敏感性。
Isolated rat peritoneal mast cells incubated in Ca-free media for 2 h, with or without EDTA, and observed by phase-contrast microscopy, became bubbled in appearance when subsequently exposed to media rich in Ca (16-110 mM). EM showed the response to be compound exocytosis of the sort elicited by conventional mast cell secretagogues such as antigen (in sensitized cells) and 48/80. The response to Ca was inhibited by withdrawing glucose and adding dinitrophenol and was thus energy-dependent. Mg in similarly high concentration had no such stimulant effect on Ca-deprived cells, and excess Ca stimulated only after Ca deprivation. Ca deprivation may increase the permeability of the plasma membrane of the mast cell thereby allowing some Ca, when subsequently introduced in high concentration, to penetrate and activate exocytosis. The results may provide further support for the postulated mediator function of Ca in stimulus-secretion coupling. Two inhibitory effects of Ca in high concentration were detected: suppression of migration or expulsion of granules from the exocytotic pits within the cellular domain; and diminished sensitivity to 48/80 [condensation product of 4-methoxy-N-methyl-benzeneethanamine and formaldehyde].