The mesoderm determinant Snail collaborates with related zinc-finger proteins to control Drosophila neurogenesis
The mesoderm determinant Snail collaborates with related zinc-finger proteins to control Drosophila neurogenesis
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DOI：
10.1093/emboj/18.22.6426
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发表时间：
1999-11-15
期刊：
影响因子：
11.4
通讯作者：
Ip, YT
中科院分区：
文献类型：
--
作者：
Ashraf, SI;Hu, XD;Ip, YT
Snail蛋白作为一种转录调节因子，在早期中胚层细胞命运的建立中发挥作用。后来，在胚带延伸的胚胎中，Snail也在大多数神经母细胞中表达。在这里，我们提出证据表明，Snail的表达是中枢神经系统（CNS）发育所必需的。蜗牛的神经功能被两个紧密相连的基因所掩盖，scargot和worniu。蜗牛和Worniu都含有与蜗牛高度同源的锌指结构域。虽然不影响早期成神经细胞标志物的表达，但包含所有三个基因的区域的缺失与CNS决定簇（包括fushi tarazu、pdm-2和even-skipped）的表达丧失相关。三种Snail家族蛋白的转基因表达均能有效地挽救fushi tarazu缺陷，部分挽救pdm-2缺陷，甚至挽救跳过的CNS模式。这些结果表明，Snail家族蛋白在胚胎CNS发育过程中，神经节母细胞形成前后具有重要功能。
The Snail protein functions as a transcriptional regulator to establish early mesodermal cell fate, Later, in germ band-extended embryos, Snail is also expressed in most neuroblasts, Here we present evidence that this expression of Snail is required for central nervous system (CNS) development. The neural function of snail is masked by two closely linked genes, escargot and worniu. Both Escargot and Worniu contain zinc-finger domains that are highly homologous to that of Snail. Although not affecting expression of early neuroblast markers, the deletion of the region containing all three genes correlates with loss of expression of CNS determinants including fushi tarazu, pdm-2 and even-skipped. Transgenic expression of each of the three Snail family proteins can rescue efficiently the fushi tarazu defects, and partially the pdm-2 and even-skipped CNS patterns. These results demonstrate that the Snail family proteins have essential functions during embryonic CNS development, around the time of ganglion mother cell formation.