Effect of axonal micro-tubules on the morphology of retinal nerve fibers studied by second-harmonic generation.
Effect of axonal micro-tubules on the morphology of retinal nerve fibers studied by second-harmonic generation.
复制标题
通过二次谐波产生研究轴突微管对视网膜神经纤维形态的影响。
DOI：
10.1117/1.jbo.17.11.110502
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发表时间：
2012
影响因子：
3.5
通讯作者：
Danias,John
中科院分区：
文献类型：
--
作者：
Lim,Hyungsik;Danias,John
许多研究表明，视网膜神经节细胞中微管的降解可能是青光眼进展的早期事件。由于视网膜神经纤维的反射和双折射主要来自微管，因此已经深入研究了用于疾病早期检测的光学特性。我们以前报道了一种新的非线性光学信号从轴突微管可视化视网膜神经纤维，即二次谐波产生（SHG）。我们证明了使用轴突SHG研究微管对视网膜神经纤维束形态的影响。在诺考达唑药物治疗期间，对活体视网膜平片进行延时SHG成像，并监测轴突SHG强度和神经纤维束形态的变化。我们发现，微管破坏不会导致神经纤维形态的立即改变。我们的研究结果表明，微管SHG可能提供一个有用的手段，敏感检测轴突损伤。由于内在辐射取决于细胞骨架元件的规则结构，如由活跃的细胞调节所维持的，信号的强度反映了视网膜神经节细胞轴突的健康。
Many studies suggest that the degradation of microtubules in the retinal ganglion cells may be an early event in the progression of glaucoma. Because reflectance and birefringence of the retinal nerve fibers arise primarily from microtubules, the optical properties have been intensively studied for early detection of the disease. We previously reported a novel nonlinear optical signal from axonal microtubules for visualizing the retinal nerve fibers, namely second-harmonic generation (SHG). We demonstrate the use of axonal SHG to investigate the effect of microtubules on the morphology of the retinal nerve fiber bundles. Time-lapse SHG imaging ofex vivorat retinal flat mounts was performed during pharmacological treatment of nocodazole, and the intensity of axonal SHG and the changes in nerve fiber bundle morphology were monitored. We found that the microtubule disruption does not lead to immediate modification in the morphology of the nerve fibers. Our results indicate that microtubular SHG may provide a useful means for sensitive detection of axonal injuries. Since the intrinsic radiation depends on the regular architecture of the cytoskeleton element as maintained by active cellular regulations, the intensity of signal reflects the health of the retinal ganglion cell axons.