Corynebacterium pyruviciproducens, as an immune modulator, can promote the activity of macrophages and up-regulate antibody response to particulate antigen
Corynebacterium pyruviciproducens, as an immune modulator, can promote the activity of macrophages and up-regulate antibody response to particulate antigen
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丙酮棒杆菌作为免疫调节剂，可以促进巨噬细胞的活性并上调对颗粒抗原的抗体反应
DOI：
10.1258/ebm.2012.012181
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发表时间：
2012-11-01
影响因子：
3.2
通讯作者：
Xu, Huaxi
中科院分区：
文献类型：
--
作者：
Tong, Jia;Han, Qingzhen;Xu, Huaxi
丙酮棒杆菌是新发现的棒状杆菌属物种，不含有白喉毒素、结核硬脂酸等已知致病成分，与痤疮丙酸杆菌具有相似的生物学特性，但其免疫调节作用正引起人们的关注。本工作基于巨噬细胞作为初级清道夫和颗粒性抗原呈递细胞清除病原菌的作用，通过检测细胞因子分泌和膜分子表达水平，分析丙酮酸梭菌对巨噬细胞的刺激作用，并检测丙酮酸梭菌在体内促进绵羊红细胞（SRBC）抗体反应的作用。在体外，丙酮酸梭菌导致白细胞介素 6 和肿瘤坏死因子 α 的急剧释放，并促进巨噬细胞的激活，包括增强 MHC-II、CD40、CD80 和 CD86 的表达。在体内，它增强了针对 SRBC（一种颗粒抗原）的体液免疫反应。这些观察结果表明，丙酮酸梭菌作为一种免疫调节剂，可以通过调节巨噬细胞功能来促进宿主对病原微生物的体液免疫反应。
Corynebacterium pyruviciproducens is a newly discovered Corynebacterium species with no known pathogenic components such as diphtheria toxin and tuberculostearic acid, and it has similar biological properties to Propionibacterium acnes, but its role of immunoregulation is drawing people's attention. In this work, based on the role of macrophages in removal of pathogenic bacteria as a primary scavenger and particulate antigen-presenting cell, the stimulation of macrophages by C. pyruviciproducens was analyzed through detecting the levels of cytokine secretion and expression of membrane molecules, and the effect of C. pyruviciproducens in promoting antibody response to sheep red blood cells (SRBC) in vivo was detected. In vitro, C. pyruviciproducens led to a sharp release of interleukin-6 and tumour necrosis factor-α and encouraged the activation of macrophages including enhanced expressions of MHC-II, CD40, CD80 and CD86. In vivo, it enhanced the humoral immune response against SRBC, a particulate antigen. These observations suggest that C. pyruviciproducens, as an immunoregulator, can promote the host humoral immune response to pathogenic microorganisms by regulating macrophage function.