Diversity in the origins of sex chromosomes in anurans inferred from comparative mapping of sexual differentiation genes for three species of the Raninae and Xenopodinae
Diversity in the origins of sex chromosomes in anurans inferred from comparative mapping of sexual differentiation genes for three species of the Raninae and Xenopodinae
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DOI：
10.1007/s10577-008-1257-z
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发表时间：
2008-10-01
影响因子：
2.6
通讯作者：
Matsuda, Yoichi
中科院分区：
文献类型：
--
作者：
Uno, Yoshinobu;Nishida, Chizuko;Matsuda, Yoichi
两栖动物采用遗传性别决定系统与男性和女性异配子。两栖动物性别决定的祖先状态被认为是雌性异配子;然而，性染色体和性别决定基因的起源仍然未知。非洲爪蟾的3号染色体上有一个性别（卵巢）决定基因（DM-W），最近被鉴定为W性染色体。本研究采用比较基因组杂交技术对黄曲藻进行了研究。非洲爪蟾和热带爪蟾的八个性分化基因的FISH定位。laevis，黄毛菊X. tropicalis和Rana rugosa。3个伴性基因。rugosa-AR、SF-1/Ad 4 BP和Sox 3-均定位于X.而在X. tropicalis中，AR和SF-1/Ad 4 BP定位于14号染色体，Sox 3定位于11号染色体。光滑。这些结果表明，W性染色体是在X的谱系中独立获得的。laevis的ZW性染色体起源不同。laevis和R.皱纹。Cyp 17、Cyp 19、Dmrt 1、Sox 9和WT 1在X染色体上定位于常染色体。laevis和R. rugosa，这表明这五个基因可能不是候选人的性别决定基因在两个无尾类物种。
Amphibians employ genetic sex determination systems with male and female heterogamety. The ancestral state of sex determination in amphibians has been suggested to be female heterogamety; however, the origins of the sex chromosomes and the sex-determining genes are still unknown. In Xenopus laevis, chromosome 3 with a candidate for the sex- (ovary-) determining gene (DM-W) was recently identified as the W sex chromosome. This study conducted comparative genomic hybridization for X. laevis and Xenopus tropicalis and FISH mapping of eight sexual differentiation genes for X. laevis, X. tropicalis, and Rana rugosa. Three sex-linked genes of R. rugosa-AR, SF-1/Ad4BP, and Sox3-are all localized to chromosome 10 of X. tropicalis, whereas AR and SF-1/Ad4BP are mapped to chromosome 14 and Sox3 to chromosome 11 in X. laevis. These results suggest that the W sex chromosome was independently acquired in the lineage of X. laevis, and the origins of the ZW sex chromosomes are different between X. laevis and R. rugosa. Cyp17, Cyp19, Dmrt1, Sox9, and WT1 were localized to autosomes in X. laevis and R. rugosa, suggesting that these five genes probably are not candidates for the sex-determining genes in the two anuran species.