ALDOSE REDUCTASE IN THE BB RAT - ISOLATION, IMMUNOLOGICAL IDENTIFICATION AND LOCALIZATION IN THE RETINA AND PERIPHERAL-NERVE
ALDOSE REDUCTASE IN THE BB RAT - ISOLATION, IMMUNOLOGICAL IDENTIFICATION AND LOCALIZATION IN THE RETINA AND PERIPHERAL-NERVE
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DOI：
10.1007/bf00270423
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发表时间：
1987-04-01
期刊：
影响因子：
8.2
通讯作者：
GREENE, DA
中科院分区：
文献类型：
--
作者：
CHAKRABARTI, S;SIMA, AAF;GREENE, DA
采用DEAE纤维素、羟基磷灰石和葡聚糖G-100柱层析纯化非糖尿病BB大鼠睾丸醛糖还原酶。分离得到的酶分子量为365,500 .+-。1000. 在家兔中培养了针对分离酶的抗体。经亲和层析纯化，双免疫扩散和Western blot鉴定，用于大鼠视网膜和周围神经的定位。在视网膜中，醛糖还原酶免疫反应性见于神经节细胞、穆勒细胞突、视网膜色素上皮以及视网膜毛细血管的周细胞和内皮细胞。在周围神经中，雪旺细胞的副神经节细胞质和神经内膜毛细血管的周细胞和内皮细胞中发现醛糖还原酶免疫反应性。
Aldose reductase was purified from testis of nondiabetic BB rats using DEAE cellulose, hydroxylapatite and sephadex G-100 column chromatography. The molecular weight of the isolated enzyme was found to be 365,500 .+-. 1000. Antibody against the isolated enzyme was raised in rabbits. It was purified by affinity chromatography, characterised by double immunodiffusion and Western blot analysis and used to localize the enzyme in retina and in peripheral nerve of the BB rat. In the retina, aldose reductase immunoreactivity was seen in the ganglion cells, Muller cell processes, retinal pigment epithelium and in the pericytes and endothelial cells of retinal capillaries. In peripheral nerve, aldose reductase immunoreactivity was found in the paranodal cytoplasm of Schwann cells and in pericytes and endothelial cells of endoneurial capillaries.