Tafazzin (TAZ) promotes the tumorigenicity of cervical cancer cells and inhibits apoptosis.
Tafazzin (TAZ) promotes the tumorigenicity of cervical cancer cells and inhibits apoptosis.
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Tafazzin (TAZ)促进宫颈癌细胞致瘤性并抑制细胞凋亡
DOI：
10.1371/journal.pone.0177171
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Zheng PS
中科院分区：
文献类型：
--
作者：
Chen M;Zhang Y;Zheng PS
Tafazzin （TAZ）常在某些癌症中异常表达，包括直肠癌和甲状腺肿瘤。然而，TAZ在宫颈癌细胞中的功能尚不清楚。本研究旨在探讨TAZ在宫颈癌细胞中的表达及功能。我们通过免疫组化检测了TAZ蛋白在正常宫颈组织（NC, n = 27）、高级别鳞状上皮内病变（HSIL, n = 26）和宫颈鳞状癌（SCC, n = 41）中的表达，从NC到HSIL再到SCC， TAZ蛋白的表达逐渐增加。通过稳定转染表达TAZ的质粒或靶向TAZ的shRNA质粒，使TAZ在宫颈癌细胞中过表达或下调。体外细胞生长曲线和MTT实验表明，TAZ可促进宫颈癌细胞的生长和活力。在体内，异种移植实验表明TAZ可以提高肿瘤形成能力。FACS和TUNEL分析的凋亡细胞百分比一致显示TAZ抑制宫颈癌细胞凋亡。此外，TAZ还下调了宫颈癌细胞中Cleaved Caspase 9和Cleaved Caspase 3。综上所述，本研究表明TAZ在宫颈癌中过表达，可能促进宫颈癌细胞的致瘤性，抑制凋亡。
Tafazzin (TAZ) is often aberrantly expressed in some cancers, including rectal cancer and thyroid neoplasms. However, the function of TAZ in cervical cancer cells remains unknown. This study aims to explore the expression and function of TAZ in cervical cancer cells. Here, we determined the expression of TAZ protein in normal cervical tissue (NC, n = 27), high-grade squamous intraepithelial lesions (HSIL, n = 26) and squamous cervical carcinoma (SCC, n = 41) by immunohistochemistry, the expression of TAZ protein gradually increased from NC to HSIL to SCC. TAZ was overexpressed or down-regulated in cervical cancer cells by stably transfecting a TAZ-expressing plasmid or a shRNA plasmid targeting TAZ. In vitro, the cell growth curves and MTT assays showed that TAZ may promote the growth and viability of cervical cancer cells. In vivo, xenografts experiment showed that TAZ may increase tumor-forming ability. The percentage of apoptosis cells analyzed by FACS and TUNEL assays consistently showed that TAZ inhibits apoptosis in cervical cancer cells. Furthermore, the Cleaved Caspase 9 and Cleaved Caspase 3 were down-regulated by TAZ in cervical cancer cells. Taken together, this study demonstrated that TAZ is overexpressed in cervical cancer and may promote tumorigenicity of cervical cancer cells and inhibit apoptosis.