Liposomes containing colloidal gold are a useful probe of liposome-cell interactions.
Liposomes containing colloidal gold are a useful probe of liposome-cell interactions.
复制标题
含有胶体金的脂质体是脂质体-细胞相互作用的有用探针。
DOI：
10.1016/0005-2736(83)90220-1
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Papahadjopoulos,D
中科院分区：
文献类型：
--
作者：
Hong,K;Friend,DS;Glabe,CG;Papahadjopoulos,D
本发明描述了一种制备含有胶体金的脂质体的方法，该脂质体作为追踪脂质体-细胞相互作用的电子密度标记物。由于金溶胶会在装载大量脂质体所需的高浓度下沉淀，因此金溶胶是在包裹氯化金的预成型脂质体中形成的。对反相蒸发法制备的脂质体，确定了标记物包封的最佳条件。金溶胶在常温下无有机溶剂快速形成，在脂质体内形成均匀的金颗粒。大多数囊泡含有标记物，使我们能够明确地确定脂质体及其含量的细胞内命运。体外实验表明，金脂质体被非洲绿猴肾细胞内化的方式类似于受体介导的配体内吞作用。初步的体内研究也表明脂质体通过包被囊泡途径被库普弗细胞内吞。
A method is described for the preparation of liposomes containing colloidal gold as an electron-dense marker to trace liposome-cell interactions. Since gold sols would precipitate at the high concentrations necessary for loading a large proportion of liposomes, gold sols were formed within preformed liposomes which had encapsulated gold chloride. The optimal conditions for encapsulating the marker were ascertained for liposomes prepared by the method of reverse-phase evaporation. Gold sols formed rapidly at ambient temperature and without organic solvent, and produced homogeneous populations of gold granules inside liposomes. Most vesicles contained the marker, allowing us to determine unambiguously the intracellular fate of liposomes and their contents. The in vitro experiments showed that gold-liposomes were internalized by African green monkey kidney cells in a manner similar to receptor-mediated endocytosis of well-characterized ligands. Preliminary in vivo studies also indicated that liposomes were endocytosed by Kupffer cells via the coated vesicle pathway.