Wogonin attenuates ovalbumin antigen-induced neutrophilic airway inflammation by inhibiting th17 differentiation.
Wogonin attenuates ovalbumin antigen-induced neutrophilic airway inflammation by inhibiting th17 differentiation.
复制标题
DOI：
10.1155/2014/571508
复制
发表时间：
2014
影响因子：
2
通讯作者：
Matsushita S
中科院分区：
文献类型：
--
作者：
Takagi R;Kawano M;Nakagome K;Hashimoto K;Higashi T;Ohbuchi K;Kaneko A;Matsushita S
过敏性气道炎症通常被认为是Th 2型免疫应答。然而，最近的研究表明，Th 17型免疫应答在这一过程中也起着重要作用，特别是在嗜酸性气道炎症的发病机制中，这是严重哮喘的标志。我们仔细检查了几个汉方提取物，据报道，通过使用人和小鼠幼稚T细胞的体外分化系统表现出抗炎活性。我们发现hange-shashin-to（HST）和oren-gedoku-to（OGT）在体外对Th 17反应具有抑制活性。事实上，汉黄芩素和小檗碱，共同的HST和OGT的主要成分，表现出Th 17抑制活性，在小鼠和人类系统在体外。因此，我们评估了汉黄芩素是否抑制OVA诱导的嗜肺气道炎症的OVA TCR转基因DO11.10小鼠。因此，口服汉黄芩素显著改善了OVA诱导的嗜酸性气道炎症。汉黄芩素抑制幼稚T细胞向Th 17细胞的分化，而对活化的Th 17细胞没有影响。
Allergic airway inflammation is generally considered to be a Th2-type immune response. Recent studies, however, have demonstrated that Th17-type immune responses also play important roles in this process, particularly in the pathogenesis of neutrophilic airway inflammation, a hallmark of severe asthma. We scrutinized several Kampo extracts that reportedly exhibit anti-inflammatory activity by using in vitro differentiation system of human and mouse naïve T cells. We found that hange-shashin-to (HST) and oren-gedoku-to (OGT) possess inhibitory activity for Th17 responses in vitro. Indeed, wogonin and berberine, major components common to HST and OGT, exhibit Th17-inhibitory activities in both murine and human systems in vitro. We therefore evaluated whether wogonin suppresses OVA-induced neutrophilic airway inflammation in OVA TCR-transgenic DO11.10 mice. Consequently, oral administration of wogonin significantly improved OVA-induced neutrophilic airway inflammation. Wogonin suppressed the differentiation of naïve T cells to Th17 cells, while showing no effects on activated Th17 cells.