The interleukin 1 receptor. Dynamics of interleukin 1 binding and internalization in T cells and fibroblasts.
The interleukin 1 receptor. Dynamics of interleukin 1 binding and internalization in T cells and fibroblasts.
复制标题
白细胞介素1受体。
DOI：
10.4049/jimmunol.138.9.2906
复制
发表时间：
1987
影响因子：
4.4
通讯作者：
R. Chizzonite
中科院分区：
文献类型：
--
作者：
S. Mizel;P. L. Kilian;J. Lewis;K. Paganelli;R. Chizzonite
在这项研究中，我们已经证明，小鼠T细胞淋巴瘤，EL 4，和小鼠成纤维细胞系，瑞士3 T3，具有一类高亲和力的白细胞介素1（IL 1）受体，存在于一个动态平衡状态，是由IL 1的影响。在没有IL 1的情况下，IL 1受体似乎以大约11小时的t1/2进行周转。然而，当细胞在IL 1存在下孵育时，IL 1受体经历广泛的配体诱导的下调。IL 1本身在37 ℃时内化; 50%的表面结合IL 1在60至120分钟内内化。IL 1在内化后至少6小时内不发生降解。通过使用电子显微镜和放射自显影，我们观察到的内化过程中的几个重要特征。当在4 ℃结合125 I-IL 1的细胞转移到37 ℃时，IL 1从细胞膜移动到细胞质，在细胞质中发现IL 1接近细胞核或在溶酶体内。IL-1在细胞核内进行性蓄积。在将细胞转移到37摄氏度后6小时，30%至35%的内化的125 I-IL 1与细胞核结合。相对高水平的IL 1在细胞核中的积累提出了一种有趣的可能性，即IL 1不仅可以以高度特异性的方式与细胞表面受体相互作用，而且还可以与潜在的重要核受体相互作用。
In this study, we have demonstrated that a murine T cell lymphoma, EL 4, and a murine fibroblast cell line, Swiss 3T3, possess a single class of high affinity interleukin 1 (IL 1) receptors that exist in a dynamic state of equilibrium that is influenced by IL 1. In the absence of IL 1, the IL 1 receptor appears to turnover with a t1/2 of approximately 11 hr. However, when cells are incubated in the presence of IL 1, the IL 1 receptor undergoes extensive ligand-induced down-regulation. IL 1 itself is internalized at 37 degrees C; 50% of the surface-bound IL 1 is internalized in 60 to 120 min. IL 1 does not undergo degradation for at least 6 hr after internalization. By using electron microscopy and autoradiography, we observed several important features of the internalization process. When cells having bound 125I-IL 1 at 4 degrees C were shifted to 37 degrees C, IL 1 moved from the cell membrane to the cytoplasm where it was found in proximity to nuclei or within lysosomes. IL 1 appeared to progressively accumulate in nuclei. Six hours after shifting cells to 37 degrees C, 30 to 35% of the internalized 125I-IL 1 is associated with the cell nucleus. The accumulation of relatively high levels of IL 1 in the nucleus raises the interesting possibility that IL 1 may not only interact in a highly specific manner with cell surface receptors, but also with potentially important nuclear receptors.