Prefrontal-enriched SLIT1 expression in Old World monkey cortex established during the postnatal development.
Prefrontal-enriched SLIT1 expression in Old World monkey cortex established during the postnatal development.
复制标题
DOI：
10.1093/cercor/bhp319
复制
发表时间：
2010-10
期刊：
影响因子：
--
通讯作者：
Yamamori T
中科院分区：
文献类型：
--
作者：
Sasaki T;Komatsu Y;Watakabe A;Sawada K;Yamamori T
为了阐明皮质结构特化的分子基础，我们通过限制性标志cDNA扫描寻找旧大陆猴新皮质区之间差异表达的基因。我们发现轴突引导分子SLIT 1的mRNA在前额叶皮质中富集，但存在与发育相关的变化。原位杂交结果显示，SLIT 1 mRNA主要分布于大脑皮层的中层，在前额叶皮层中表达较强，在初级感觉区中表达较弱。主要视区表达最低。对其他SLIT（SLIT 2和SLIT 3）mRNA的分析显示，在前额叶皮层中优先表达，具有明显的层状模式。相比之下，受体Roundabout（ROBO 1和ROBO 2）mRNA广泛分布在整个皮层。在围产期，SLIT 1 mRNA在皮质中大量表达，具有适度的区域特异性。表达下调最初发生在出生后第60天左右的早期感觉区，随后在联合区。前额叶区富集SLIT 1 mRNA表达的结果，从一个相对较大的衰减，这种表达在其他地区。这些结果表明，它的作用是改变出生后，这是特别重要的前额叶连接在旧世界的猴子皮层。
To elucidate the molecular basis of the specialization of cortical architectures, we searched for genes differentially expressed among neocortical areas of Old World monkeys by restriction landmark cDNA scanning . We found that mRNA of SLIT1, an axon guidance molecule, was enriched in the prefrontal cortex but with developmentally related changes. In situ hybridization analysis revealed that SLIT1 mRNA was mainly distributed in the middle layers of most cortical areas, robustly in the prefrontal cortex and faintly in primary sensory areas. The lowest expression was in the primary visual area. Analyses of other SLIT (SLIT2 and SLIT3) mRNAs showed preferential expression in the prefrontal cortex with a distinct laminar pattern. By contrast, the receptor Roundabout (ROBO1 and ROBO2) mRNAs were widely distributed throughout the cortex. Perinatally, SLIT1 mRNA was abundantly expressed in the cortex with modest area specificity. Downregulation of expression initially occurred in early sensory areas around postnatal day 60 and followed in the association areas. The prefrontal area–enriched SLIT1 mRNA expression results from a relatively greater attenuation of this expression in the other areas. These results suggest that its role is altered postnatally and that this is particularly important for prefrontal connectivity in the Old World monkey cortex.
登录
查看更多内容
影响因子：
64.5
作者：
Brose, K;Bland, KS;Kidd, T
通讯作者：
Kidd, T
影响因子：
4.6
作者：
Andrews, William;Liapi, Anastasia;Richards, Linda J.
通讯作者：
Richards, Linda J.
影响因子：
2.5
作者：
FITZPATRICK, D;LUND, JS;TOWLES, AC
通讯作者：
TOWLES, AC
影响因子：
3.7
作者：
Batardière, A;Barone, P;Kennedy, H
通讯作者：
Kennedy, H
影响因子：
3.7
作者：
Felleman, Daniel J.;Van Essen, David C.
通讯作者：
Van Essen, David C.