Monitoring Protein Endocytosis and Recycling Using FACS-Based Assays.
Monitoring Protein Endocytosis and Recycling Using FACS-Based Assays.
复制标题
使用基于 FACS 的测定法监测蛋白质内吞作用和回收。
DOI：
10.1007/978-1-4939-9450-2_20
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Roche,PaulA
中科院分区：
文献类型：
--
作者：
Walseng,Even;Roche,PaulA
已知细胞表面 MHC II 类 (MHC-II) 会内化并可从抗原呈递细胞的内体循环回质膜。我们现在描述一种简单的方案，允许人们跟踪细胞表面蛋白质的内化动力学。此外，对该测定法的简单修改允许人们监测内化蛋白质回到质膜的出现率。该测定甚至可以对稀有细胞亚群上的蛋白质运输进行定量，这是传统生化测定无法实现的。
Cell surface MHC class II (MHC-II) is known to internalize and can recycle back to the plasma membrane from endosomes in antigen presenting cells. We now describe a simple protocol that allows one to follow the internalization kinetics of proteins from the surface of cells. Furthermore, a simple adaptation of this assay allows one to monitor the rate of appearance of internalized proteins back to the plasma membrane. This assay allows for quantitation of trafficking of proteins on even rare subsets of cells, something that is not possible with traditional biochemical assays.