Effects of strontium ranelate treatment on osteoblasts cultivated onto scaffolds of trabeculae bovine bone
Effects of strontium ranelate treatment on osteoblasts cultivated onto scaffolds of trabeculae bovine bone
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DOI：
10.1007/s00774-017-0822-y
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发表时间：
2018-01-01
影响因子：
3.3
通讯作者：
Jorge, Erika Cristina
中科院分区：
文献类型：
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作者：
Borges Silva, Gerluza Aparecida;Bertassoli, Bruno Machado;Jorge, Erika Cristina
牛骨块作为促进骨再生的植入性支架已显示出良好的效果。雷奈酸锶(SRR)既是一种抗吸收药物，也是一种合成代谢药物，已被推荐口服治疗骨质疏松症。然而，到目前为止，很少有研究调查SRR的局部影响及其与生物材料的结合使用。在这项工作中，我们研究了原代成骨细胞(PO，来自Wistar大鼠颅骨)和永生化成骨细胞(IO，来自MC3T3-E1细胞系)作为单层培养或与矿化(MBB)和脱矿(DBB)牛骨支架联合培养的SRR效应。诱导SRR对细胞活力影响的最佳剂量为0.1 mM。我们的结果表明，SRR的局部给药是生物相容的，无细胞毒性。此外，SRR似乎通过增强碱性磷酸酶活性、成骨分化标志物的表达、有机基质的合成以及矿化结节中钙离子的减少来促进原代成骨细胞的分化。DBB是促进PO和IO细胞增殖的较好支架材料。暴露脱钙骨基质中的蛋白质可能会改善支架的骨传导性能。我们的结果表明了进一步研究SRR在骨修复部位给药的重要性。SRR与骨移植的相关性表明，SRR有可能作为骨组织生物工程和骨再生治疗的辅助佐剂。
Blocks of Bovine bone have shown promising results as implantable scaffolds to promote bone regeneration. Strontium ranelate (SrR) is both an antiresorptive and an anabolic drug that has been indicated for oral administration to treat osteoporosis. Few studies, however, have investigated the local effects of SrR and its use in association with biomaterials thus far. In this work, we investigated SrR effects in cultures of primary osteoblasts (PO, from Wistar rats calvaria) and immortalized osteoblasts (IO, from MC3T3-E1 cell line) cultivated as a monolayer or in association with scaffolds of bovine bone in mineralized (MBB) and demineralized (DBB) forms. The optimum dose to induce SrR effects on cell viability was established as 0.1 mM. Our results suggested that the local administration of SrR is biocompatible and non-cytotoxic. In addition, SrR appeared to accelerate primary osteoblast cell differentiation by enhancing alkaline phosphatase activity, the expression of osteogenic differentiation markers, the synthesis of the organic matrix, and a decrease of Ca2+ ions in mineralized nodules. DBB was found to be a better scaffold material to promote PO and IO cell proliferation. Exposing the proteins of the demineralized bone matrix might improve scaffold osteoconductive properties. Our results indicated the importance of further investigation of the administration of SrR at sites of bone repair. The association of SrR and bone grafts suggests the possibility of using SrR as a co-adjuvant for bone tissue bioengineering and in bone regeneration therapies.