Age-Specific Reference Ranges for Serum Testosterone and Androstenedione Concentrations in Women Measured by Liquid Chromatography-Tandem Mass Spectrometry
Age-Specific Reference Ranges for Serum Testosterone and Androstenedione Concentrations in Women Measured by Liquid Chromatography-Tandem Mass Spectrometry
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DOI：
10.1210/jc.2011-2134
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发表时间：
2012-02-01
影响因子：
5.8
通讯作者：
Brabant, Georg
中科院分区：
文献类型：
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作者：
Haring, Robin;Hannemann, Anke;Brabant, Georg
目的：基于RIA的性激素测量在低浓度范围的女性中仅提供有限的精确度和特异性。因此，我们的目的是建立特定年龄的参考范围，血清性激素浓度的妇女使用质谱和分位数region.Methods和结果：数据从985名妇女年龄在20-80岁，招募的前瞻性研究健康在波美拉尼亚，包括在分析。进行分位数回归模型计算女性性激素浓度的年龄特异性第2.5和第97.5分位数。采用液相色谱-串联质谱法测定血清总睾酮（TT）和雄烯二酮（AD）浓度。测量的SHBG和TT浓度用于计算游离睾酮（游离T）。TT、AD和游离T浓度在10岁年龄组中显示出明显的年龄相关性下降（单因素方差分析P < 0.001）。TT、AD和游离T的性激素参考范围在每个年龄和10岁年龄组中确定。在20-80岁的整个年龄范围内，TT的参考范围为0.35-1.97 nmol/L，AD的参考范围为0.89-4.56 nmol/L，游离T的参考范围为0.0025-0.0253 nmol/L。为绝经前和绝经后妇女以及纳入口服避孕药或激素治疗的妇女提供了单独的参考范围（n = 1357）。结论：这是第一项建立年龄特异性参考范围的研究，用于液相色谱-串联质谱法测量TT和AD，并基于分位数回归分析计算游离T浓度，准确地解释了观察到的低浓度范围和这些性激素在女性中的强烈年龄依赖性。（J Clin Endocrinol Metab 97：408-415，2012）
Objective: RIA-based sex hormone measurements offer only limited precision and specificity in the low concentration range of women. Therefore, we aimed to establish age-specific reference ranges for serum sex hormone concentrations in women using mass spectrometry and quantile regression.Methods and Results: Data from 985 women aged 20-80 yr, recruited for the prospective Study of Health in Pomerania, were included in the analyses. Quantile regressions models were performed to calculate the age-specific 2.5th and 97.5th percentiles for sex hormone concentrations in women. Serum total testosterone (TT) and androstenedione (AD) concentrations were measured by liquid chromatography-tandem mass spectrometry. Measured concentrations of SHBG and TT were used to calculate free testosterone (free T). TT, AD, and free T concentrations showed a distinct age-related decline across 10-yr age groups (one way ANOVA P < 0.001). Sex hormone reference ranges for TT, AD, and free T were determined across each single year of age and for 10-yr age groups. Reference ranges over the whole age range of 20-80 yr were 0.35-1.97 nmol/liter for TT, 0.89-4.56 nmol/liter for AD, and 0.0025-0.0253 nmol/liter for free T. Separate reference ranges were provided for pre- and postmenopausal women as well as after inclusion of women using oral contraceptives or hormone therapy (n = 1357).Conclusion: This is the first study to establish age-specific reference ranges for liquid chromatography-tandem mass spectrometry-measured TT and AD and calculated free T concentrations based on quantile regression analyses, accurately accounting for the observed low concentration range and the strong age dependency of these sex hormones in women. (J Clin Endocrinol Metab 97: 408-415, 2012)