BMP-2-embedded atelocollagen scaffold for tissue-engineered cartilage cultured in the medium containing insulin and Triiodothyronine-A new protocol for 3D in vitro culture of human chondrocytes
BMP-2-embedded atelocollagen scaffold for tissue-engineered cartilage cultured in the medium containing insulin and Triiodothyronine-A new protocol for 3D in vitro culture of human chondrocytes
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BMP-2 嵌入的去端胶原支架用于在含有胰岛素和三碘甲腺原氨酸的培养基中培养的组织工程软骨 - 人软骨细胞 3D 体外培养的新方案
DOI：
10.1089/ten.tec.2011.0217
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发表时间：
2012
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et al.
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作者：
Ko EC;et al.
当软骨细胞从天然软骨中分离并在体外增殖时，它们很快失去其表达糖胺聚糖（GAG）和II型胶原的原始能力，这被称为去分化，或降低细胞活力。我们首先研究了由人耳廓软骨细胞和端胶原组成的组织工程颗粒的体外软骨再生，并在体外用骨形态发生蛋白-2（BMP-2）、胰岛素和T3刺激下孵育。然后，我们研究了这些生长因子的管理到支架或在介质中，并探讨了可能性，即去端胶原，软骨细胞的水凝胶支架，可能功能的药物输送的因素。在培养基中添加胰岛素和T3的情况下，去端胶原中的BMP-2不仅可以在较短的时间内产生更大的GAG基质，而且可以维持细胞活力，降低死亡率。在培养基中的胰岛素可以更好地仅施用2周，而不是3周，这将节省时间和成本，从而缩短软骨细胞的体外培养。将BMP-2与去端胶原混合，并辅以胰岛素和T3激素的方法，可能为组织工程软骨的发展提供新的思路。
When the chondrocytes are isolated from the native cartilage and proliferate in vitro, they soon lose their original ability to express glycosaminoglycan (GAG) and type II collagen, which is termed dedifferentiation, or decrease cell viability. We first examined in vitro cartilage regeneration of tissue-engineered pellets that consisted of human auricular chondrocytes and atelocollagen and that were incubated in vitro under stimulation with bone morphogenetic protein-2 (BMP-2), insulin, and T3. We then examined the administration of those growth factors into the scaffold or in the medium and explored the possibility that the atelocollagen, the hydrogel scaffold of the chondrocytes, may function for drug delivery of the factors. BMP-2 in the atelocollagen with the supplement of insulin and T3 in the medium could not only produce a greater GAG matrix in a shorter period but also sustain cell viability with lower mortality. The insulin in the medium could be better administered only for 2 weeks, rather than 3 weeks, which would save time and cost, hence shortening the in vitro culture of chondrocytes. Our protocol of mixing BMP-2 into the atelocollagen with the supplement of insulin and T3 hormone might provide a new insight into the development of tissue engineering in chondrogenesis.