Therapeutic efficacy of Bifidobacterium longum‐mediated human granulocyte colony‐stimulating factor and/or endostatin combined with cyclophosphamide in mouse‐transplanted tumors
Therapeutic efficacy of Bifidobacterium longum‐mediated human granulocyte colony‐stimulating factor and/or endostatin combined with cyclophosphamide in mouse‐transplanted tumors
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DOI：
10.1111/j.1349-7006.2009.01275.x
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发表时间：
2009-10
期刊：
影响因子：
5.7
通讯作者：
Li-ping Zhu;Yan Yin;Jing Xing;Chen Li;L. Kou;Bi Hu;Zhi-wei Wu;Jian-jun Wang;Gen-xing Xu
中科院分区：
文献类型：
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作者：
Li-ping Zhu;Yan Yin;Jing Xing;Chen Li;L. Kou;Bi Hu;Zhi-wei Wu;Jian-jun Wang;Gen-xing Xu
粒细胞集落刺激因子（GCSF）常用作肿瘤化疗的促凋亡剂。长双歧杆菌（B. longum）由于其增强免疫力和在实体瘤中的选择性定位而引起研究者的兴趣。B。longum‐ pBV 22210 ‐endostatin（Endo）在我们前期的研究中被证实对肿瘤生长有明确的抑制作用。在本研究中，我们评估了B的影响。longum-pBV 22210-GCSF和/或B。longum-pBV 22210-Endo联合环磷酰胺（CTX）对H22和S180荷瘤小鼠的作用。在前期工作的基础上，构建了质粒pBV 22210-GCSF，并通过电穿孔转化到B中。longum。B。longum-pBV 22210-GCSF和/或B。longum-pBV 22210-Endo联合CTX治疗H22和S180荷瘤小鼠。治疗后进行白细胞计数，计算肿瘤抑制率。本研究采用CTX联合B. longum-pBV 22210-GCSF与B联合应用能显著提高荷瘤小鼠的白细胞水平。longum-pBV 22210-GCSF单独或B。longum-pBV 22210-Endo单独与CTX组合抑制肿瘤生长超过65%。结果表明，B. longum-pBV 22210-GCSF能有效拮抗CTX对骨髓的抑制作用，与B联合应用能抑制肿瘤生长。longum-pBV 22210-Endo和CTX。我们的研究结果提供了一个增强的理解B。longum和GCSF的研究进展以及它们作为肿瘤基因治疗的一种有效方法的潜力。（Cancer Sci 2009; 100：1986-1990）
Granulocyte colony‐stimulating factor (GCSF) is frequently used as an adjunctive agent in tumor chemotherapy. Bifidobacterium longums (B. longum) attracted researchers’ interests due to its enhancement of immunity and selective location in solid tumors. B. longum‐pBV22210‐endostatin (Endo) was proved to have a definite inhibitive effect on tumor growth in our previous study. In the present study, we evaluated the effects of B. longum‐pBV22210‐GCSF and/or B. longum‐pBV22210‐Endo combined with cyclophosphamide (CTX) on H22 and S180 tumor‐bearing mice. Based on our previous work, the plasmid pBV22210‐GCSF was constructed and transformed by electroporation into B. longum. The B. longum‐pBV22210‐GCSF and/or B. longum‐pBV22210‐Endo combined with CTX were applied to treat H22 and S180 tumor‐bearing mice. A leukocyte count was carried out and the tumor inhibition rate was calculated after treatment. In our study, CTX combined with B. longum‐pBV22210‐GCSF significantly raised the leukocyte level of tumor‐bearing mice, while combined with B. longum‐pBV22210‐GCSF alone or B. longum‐pBV22210‐Endo alone combinations with CTX inhibited tumor growth by over 65%. The results showed that B. longum‐pBV22210‐GCSF had an effective antagonistic effect on bone marrow inhibited by CTX and could inhibit tumor growth when it was combined with B. longum‐pBV22210‐Endo and CTX. Our results provide an enhanced understanding of B. longum and GCSF as well as their potential as an adjunctive approach in cancer gene therapy. (Cancer Sci 2009; 100: 1986–1990)