TRANSPLACENTAL TRANSMISSION OF A LEUKEMOGENIC MURINE LEUKEMIA-VIRUS
TRANSPLACENTAL TRANSMISSION OF A LEUKEMOGENIC MURINE LEUKEMIA-VIRUS
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DOI：
10.1128/jvi.67.10.6105-6109.1993
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发表时间：
1993-10-01
影响因子：
5.4
通讯作者：
HOPPE, PC
中科院分区：
文献类型：
--
作者：
BEDIGIAN, HG;SHEPEL, LA;HOPPE, PC
重组近交系BXH-2小鼠在生命早期自发产生B嗜性小鼠白血病病毒（MuLV），并具有自发性髓性白血病的高发病率。这些性状不是祖先品系（C57 BL/6 J和C3 H/HeJ）或其它11个重组近交BXH品系的特征。基因分析表明，该病毒不通过生殖系传播，这表明该病毒通过水平传播从一代传到下一代。一个额外的亲嗜性前病毒基因座检测到在一些小鼠的这个品系经过几代的近亲繁殖。我们表明，BXH亲嗜性病毒传播到其他菌株时，培养在病毒血症BXH-2小鼠，这些小鼠继续发展造血来源的肿瘤。我们早期发现病毒在妊娠早期表达，这表明亲嗜性MuLV也在子宫内传播。为了确定亲嗜性MuLV在子宫内传播的阶段，将植入前阶段胚胎转移到受体亲嗜性病毒阴性小鼠的子宫中。发现这些小鼠对亲嗜性MuLV的存在呈阴性，表明亲嗜性病毒的经胎盘传播容易发生在BXH-2小鼠中。尽管其他病毒（包括人慢病毒）可通过胎盘屏障传播，但MuLV经胎盘传播是罕见事件。因此，BXH-2小鼠品系可能有助于我们了解经胎盘传播和发病机制，并提供了一个潜在的新模型，用于药物治疗外源性传播的病毒相关的慢病毒。
Recombinant inbred BXH-2 mice spontaneously produce a B-tropic murine leukemia virus (MuLV) beginning early in life and have a high incidence of spontaneous myeloid leukemia. These traits are not characteristic of the progenitor strains (C57BL/6J and C3H/HeJ) or of 11 other recombinant inbred BXH strains. Genetic analysis has shown that the virus is not transmitted through the germ line, suggesting that the virus is passed from one generation to the next by horizontal transmission. An additional ecotropic proviral locus was detected in some mice of this strain after several generations of inbreeding. We show that BXH ecotropic virus was transmitted to other strains when fostered on viremic BXH-2 mice and that these mice go on to develop tumors of hematopoietic origin. Our earlier finding that virus is expressed early in gestation suggested that the ecotropic MuLV is also transmitted in utero. In order to determine the stage at which the ecotropic MuLV is transmitted in utero, preimplantation stage embryos were transferred to the uteri of recipient ecotropic virus-negative mice. These mice were found to be negative for the presence of the ecotropic MuLV, suggesting that transplacental transmission of the ecotropic virus readily occurs in BXH-2 mice. Although other viruses, including human lentiviruses, are transmitted across the placental barrier, transplacental transmission of MuLV is a rare event. Thus, the BXH-2 mouse strain may contribute to our understanding of the mechanism of transplacental transmission and pathogenesis and offers a potential new model for use in drug therapy of exogenously transmitted viruses related to lentiviruses.