Overexpression of neuropeptide Y in the dorsomedial hypothalamus increases trial initiation but does not significantly alter concentration-dependent licking to sucrose in a brief-access taste test.
Overexpression of neuropeptide Y in the dorsomedial hypothalamus increases trial initiation but does not significantly alter concentration-dependent licking to sucrose in a brief-access taste test.
复制标题
下丘脑背内侧神经肽 Y 的过度表达会增加试验的启动，但不会显着改变短暂访问味觉测试中对蔗糖的浓度依赖性舔舐。
DOI：
10.1016/j.physbeh.2013.01.003
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发表时间：
2013
影响因子：
2.9
通讯作者：
Moran,TimothyH
中科院分区：
文献类型：
--
作者：
Treesukosol,Yada;Bi,Sheng;Moran,TimothyH
文献中的证据提出了这样一种可能性：下丘脑背内侧 (DMH) 中神经肽 Y (NPY) 的改变可能会导致食欲亢进，从而导致体重增加。此前，我们已经证明，与 AAVGFP 对照相比，腺相关病毒 (AAV) 介导的瘦大鼠 DMH 中 NPY 过度表达导致体重增加显着增加，这是由于食物摄入量增加，而高脂肪饮食进一步加剧了这种情况。在这里，我们在自由采食和部分食物和水获取条件下，通过简短的味觉程序（10 秒试验，30 分钟）对 AAVNPY 和 AAVGFP 对照大鼠进行了一系列蔗糖浓度的测试。该测试通过提供试验启动（食欲）和每个浓度的无条件舔（完成）的测量，允许对行为成分进行一定程度的分离。与之前的研究结果一致，表明 NPY 对食欲功能具有主要影响，DMH NPY 的过度表达并没有显着改变对蔗糖的浓度依赖性舔舐反应，但在非限制性食物和水计划中进行测试时，与 AAVGFP 对照组相比，在简短的味觉测试中，AAVNPY 大鼠启动了明显更多的蔗糖试验。
Evidence in the literature raises the possibility that alterations in neuropeptide Y (NPY) in the dorsomedial hypothalamus (DMH) may contribute to hyperphagia leading to body weight gain. Previously, we have shown that compared to AAVGFP controls, adeno-associated virus (AAV)-mediated overexpression of NPY in the DMH of lean rats resulted in significantly higher body weight gain that was attributed to increased food intake, and this was further exacerbated by a high-fat diet. Here, we tested AAVNPY and AAVGFP control rats in a brief-access taste procedure (10-s trials, 30-min sessions) to an array of sucrose concentrations under ad libitum and partial food and water access conditions. The test allows for some segregation of the behavioral components by providing a measure of trial initiation (appetitive) and unconditioned licks at each concentration (consummatory). Consistent with previous findings suggesting that NPY has a primary effect on appetitive function, overexpression of DMH NPY did not significantly alter concentration-dependent licking response to sucrose but when tested in a non-restricted food and water schedule, AAVNPY rats initiated significantly more sucrose trials compared to AAVGFP controls in a brief-access taste test.