A novel cecropin B-derived peptide with antibacterial and potential anti-inflammatory properties.
A novel cecropin B-derived peptide with antibacterial and potential anti-inflammatory properties.
复制标题
一种具有抗菌和潜在抗炎特性的新型天蚕素 B 衍生肽
作者：
Wang J;Ma K;Ruan M;Wang Y;Li Y;Fu YV;Song Y;Sun H;Wang J
抗菌肽是一类阳离子抗菌肽，最初发现于昆虫体内。大多数丝素具有两性亲和的n端段和大部分疏水的c端段，通常形成螺旋-铰链-螺旋结构。本研究通过删除柞蚕蛾cecropin B的铰链区（丙氨酸-甘氨酸-脯氨酸），获得了一种新的32残基cecropin-like peptide cecropin DH。天蚕素DH具有有效的抗菌活性，特别是对革兰氏阴性菌，对哺乳动物细胞具有非常低的细胞毒性。cecropin DH与革兰氏阴性细菌外膜的高阴离子脂多糖（LPS）组分之间的相互作用表明，它能够解离LPS胶束，并将LPS聚集物破坏成更小的集合，这可能在其抗菌活性中发挥重要作用。利用lps刺激的小鼠巨噬细胞RAW264.7细胞，我们发现天蚕素DH比天蚕素B具有更高的潜在抗炎活性，这可以通过抑制促炎细胞因子一氧化氮的产生和肿瘤坏死因子-α的分泌来证明。综上所述，天蚕素DH具有作为抗菌和抗炎治疗药物的潜力。
Cecropins, originally found in insects, are a group of cationic antimicrobial peptides. Most cecropins have an amphipathic N-terminal segment and a largely hydrophobic C-terminal segment, and normally form a helix-hinge-helix structure. In this study, we developed the novel 32-residue cecropin-like peptide cecropin DH by deleting the hinge region (Alanine-Glycine-Proline) of cecropin B isolated from Chinese oak silk moth, Antheraea pernyi. Cecropin DH possesses effective antibacterial activity, particularly against Gram-negative bacteria, with very low cytotoxicity against mammalian cells. Interactions between cecropin DH and the highly anionic lipopolysaccharide (LPS) component of the Gram-negative bacterial outer membrane indicate that it is capable of dissociating LPS micelles and disrupting LPS aggregates into smaller assemblies, which may play a vital role in its antimicrobial activity. Using LPS-stimulated mouse macrophage RAW264.7 cells, we found that cecropin DH exerted higher potential anti-inflammatory activity than cecropin B, as demonstrated by the inhibition of pro-inflammatory cytokines nitric oxide production and secretion of tumor necrosis factor-α. In conclusion, cecropin DH has potential as a therapeutic agent for both antibacterial and anti-inflammatory applications.