Co-circulation of coxsackieviruses A6 and A10 in hand, foot and mouth disease outbreak in Finland
Co-circulation of coxsackieviruses A6 and A10 in hand, foot and mouth disease outbreak in Finland
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DOI：
10.1016/j.jcv.2010.02.002
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发表时间：
2010-05-01
影响因子：
8.8
通讯作者：
Roivainen, Merja
中科院分区：
文献类型：
--
作者：
Blomqvist, Soile;Klemola, Paivi;Roivainen, Merja
背景：2008年秋季，芬兰发生了全国范围的手足口病疫情。由于相当多的临床诊断患者是成年人，因此这次疫情并不典型。此外，许多患者在手足口病急性期后数周内发生脱甲。目的：检测、鉴定引起此次疫情的人肠道病毒（HEV）并进行系统发育分析。通过病毒分离和/或直接实时RT-PCR分析标本中HEV的存在;通过分子方法对选定的HEV毒株进行进一步分型。HEV毒株的遗传相似性进行了评估，通过系统发育分析的部分VP 1 sequence.Results：戊型肝炎病毒检测到212手足口病病例，包括儿童和成人，在整个芬兰。两种戊型肝炎病毒，柯萨奇病毒A6（CV-A6）和A10（CV-A10），被确定为爆发的病原体。一个基因变异的CV-A6占主导地位，但是，另外，其他三个不同的基因CV-A6菌株被发现。所有CV-A10菌株分离成一个基因簇不同，从以前报道的CV-A10 sequences.Conclusions：芬兰2008年手足口病爆发是由两个罕见的检测，共循环科萨基A病毒。我们的数据表明，在北方欧洲的地方性传播的CV-A型和爆发是由于这些病毒的新的遗传变异的出现。(C)2010爱思唯尔有限公司版权所有。
Background: A nationwide outbreak of hand, foot and mouth disease (HFMD) occurred in Finland in autumn 2008. The outbreak was untypical since a considerable number of clinically diagnosed patients were adults. Furthermore, many of the patients suffered from onychomadesis several weeks after the acute phase of HFMD.Objectives: Detection, identification and phylogenetic analysis of human enteroviruses (HEV) that caused the outbreak.Study design: A total of 420 clinical specimens were obtained from 317 HFMD cases all over the country. The presence of HEV in the specimens was analysed by virus isolation and/or direct real-time RT-PCR; selected HEV strains were further typed by molecular methods. The genetic similarities of HEV strains were assessed by phylogenetic analyses on partial VP1 sequences.Results: HEV were detected in 212 HFMD cases, including both children and adults, throughout Finland. Two HEV types, coxsackieviruses A6 (CV-A6) and A10 (CV-A10), were identified as the causative agents of the outbreak. One genetic variant of CV-A6 predominated, but, additionally, three other genetically distinct CV-A6 strains were found. All CV-A10 strains segregated into one genetic cluster distinct from previously reported CV-A10 sequences.Conclusions: The Finnish 2008 HFMD outbreak was caused by two infrequently detected, co-circulating, coxsackie A viruses. Our data suggest endemic circulation of both CV-A types in Northern Europe and that the outbreak was due to the emergence of new genetic variants of these viruses. (C) 2010 Elsevier B.V. All rights reserved.