Separation of selenium, zinc, and copper compounds in bovine whey using size exclusion chromatography linked to inductively coupled plasma mass spectrometry
Separation of selenium, zinc, and copper compounds in bovine whey using size exclusion chromatography linked to inductively coupled plasma mass spectrometry
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DOI：
10.1021/jf070169x
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发表时间：
2007-05-16
影响因子：
6.1
通讯作者：
Akesson, Bjorn
中科院分区：
文献类型：
--
作者：
Hoac, Tien;Lundh, Thomas;Akesson, Bjorn
为研究微量元素对牛乳品质和营养价值的影响，采用体积排阻色谱-电感耦合等离子体质谱（SEC-ICP-MS）联用技术，对乳清中硒、锌、铜的分布进行了研究。检测到硒的三个主峰、锌的两个峰和铜的五个峰。超过65%的硒存在于蛋白质组分中，主要是与主要乳清蛋白β-乳球蛋白和α-乳白蛋白相一致的组分。所有锌与低分子量化合物（< 5 kDa）相关，其中一种化合物可能是柠檬酸盐。超过60%的铜在蛋白质组分中洗脱，五个主峰中的两个可能含有金属硫蛋白和柠檬酸盐。这种方法被用来比较牛奶和乳清生产的有机和传统的喂养程序。采用有机方案生产的乳清和脱盐乳中硒含量显著低于常规样品。有机乳清中硒在蛋白质组分中的比例显著低于普通乳清，而锌和铜在蛋白质组分中的分布差异不大。本研究表明，与SEC-ICP-MS技术的几种微量元素在乳清中的分布曲线可以在同一个运行和有机和常规程序生产的乳清中不同的硒配置文件进行了研究。
To study the role of trace elements for the quality and nutritional value of bovine milk, the distribution of selenium, zinc, and copper in whey was investigated using a method linking size exclusion chromatography to inductively coupled plasma mass spectrometry (SEC-ICP-MS). Three major peaks were detected for selenium, two peaks for zinc, and five peaks for copper. More than 65% of the selenium was found in protein fractions, mainly in fractions coinciding with the major whey proteins beta-lactoglobulin and alpha-lactalbumin. All zinc was associated with low molecular weight compounds (< 5 kDa) and one of these compounds was probably citrate. More than 60% of the copper eluted in protein fractions and two of the five major peaks probably contained metallothionein and citrate. This method was used to compare milk and whey produced by organic and conventional feeding procedures. The selenium content in whey and desalted milk produced using organic regimens was significantly lower than that in conventional samples. Moreover, the proportion of selenium in protein fractions of organic whey was significantly smaller than that in conventional whey, but the distributions of zinc and copper did not differ. This study showed that with the SEC-ICP-MS technique the distribution profiles of several trace elements in whey could be studied in the same run and that the selenium profile differed in whey produced by organic and conventional procedures.